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T1763

Sigma-Aldrich

Trypsin Agarose

buffered aqueous suspension, from bovine pancreas trypsin

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About This Item

Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
42020142
NACRES:
NA.54

92,30 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Origine biologica

bovine pancreas (trypsin)

Stato

buffered aqueous suspension

Concentrazione

≥15 units/mL (packed gel)

Grado di funzionalizzazione

≥15 units per mL packed gel

Matrice

cross-linked beaded agarose

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Trypsin Agarose is an insoluble enzyme product. It is produced by reacting a conventional "soluble" enzyme (trypsin) with an inert base (agarose). This insoluble conjugate retains the activity of the original enzyme. Trypsin bound to agarose are highly stable and maintain denaturing conditions for longer time than the soluble trypsin.[1]

Applicazioni

A very active and very stable trypsin agarose derivative has been used to optimize the design of the synthesis of a model dipeptide, benzoylarginine leucinamide. [2] Trypsin has also been used in a study to investigate protonation-state determination in proteins using high-resolution X-ray crystallography. [3]
Trypsin Agarose has been used for enzymatic hydrolysis of prolamins[4][5] and gliadin[5] to generate peptides.

Altre note

Insolubilized

Definizione di unità

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).

Stato fisico

Suspension in approx. 10 mM acetic acid, pH 3.2

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Acta crystallographica. Section D, Biological crystallography, 68(Pt 7), 800-809 (2012-07-04)
A bond-distance analysis has been undertaken to determine the protonation states of ionizable amino acids in trypsin, subtilisin and lysozyme. The diffraction resolutions were 1.2 Å for trypsin (97% complete, 12% H-atom visibility at 2.5σ), 1.26 Å for subtilisin (100% complete, 11%
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