This product is soluble in 1mM HCL.
T6567
Tripsina
Proteomics Grade, BioReagent, Dimethylated
Sinonimo/i:
Porcine Trypsin, Trypsin for Mass Spectropetry
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20 μG
50,30 €
5 X 20 μG
184,00 €
1 MG
877,00 €
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Cambia visualizzazione
20 μG
50,30 €
5 X 20 μG
184,00 €
1 MG
877,00 €
About This Item
Prodotti consigliati
Origine biologica
Porcine pancreas
Livello qualitativo
Nome Commerciale
BioReagent
Solubilità
1 mM HCl: soluble 1 mg/mL, clear, colorless
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
La tripsina è una serin-proteasi impiegata generalmente in biochimica e in biologia come reagente enzimatico di rilievo.[1] Produciamo una tripsina con elevato grado di purezza, adatto per la ricerca in proteomica. Il grado per proteomica è particolarmente adatto per un impiego nelle digestioni triptiche in soluzione e in gel. La tripsina, una serina proteasi, è presente nell′apparato digerente di numerosi vertebrati.[2]
Applicazioni
La tripsina di pancreas porcino è usata per le seguenti applicazioni:
- Digestione in gel delle proteine e analisi con spettrometria di massa MALDI-TOF[3]
- Analisi con spettrometria di massa con ionizzazione per elettronebulizzazione (ESI-MS)[4]
- Profilazione del proteoma di superficie di L. plantarum[5]
- Filtrazione su gel, ultracentrifugazione e microscopia elettronica con rotary shadowing[6]
- Spettrometria di massa[7][8]
Azioni biochim/fisiol
Grazie alla sua capacità di rompere i legami in modo altamente specifico, che porta a un numero ridotto di peptidi triptici, la tripsina è utilizzata su base regolare nella ricerca proteomica in applicazioni di sequenziamento delle proteine e di mappatura dei peptidi. La tripsina idrolizza i legami peptidici solo a livello del gruppo α-carbossilico residui di arginina e lisina[2]. L′enzima mostra anche attività di esterasi e amidasi.[9] La tripsina funge da strumento per colture cellulari. L′enzima viene impiegato per idrolizzare le proteine allergeniche del latte e produrre latte ipoallergenico su scala industriale.[1]
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organi bersaglio
Respiratory system
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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I clienti hanno visto anche
Daniela M Remus et al.
Journal of bacteriology, 195(3), 502-509 (2012-11-24)
Sortases are transpeptidases that couple surface proteins to the peptidoglycan of Gram-positive bacteria, and several sortase-dependent proteins (SDPs) have been demonstrated to be crucial for the interactions of pathogenic and nonpathogenic bacteria with their hosts. Here, we studied the role
Masayuki Shimoda et al.
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Porcine islets are considered to be a promising resource for xenotransplantation. However, it is difficult to isolate porcine islets because of the marked fragility and rapid dissociation. Endogenous trypsin is one of the main factors to damage islets during the
Biomarkers of acute and chronic pancreatitis
Kaphalia BS
Biomarkers in Toxicology, 279-289 (2014)
Pauline Floch et al.
Gut pathogens, 6, 20-20 (2014-07-06)
A gamma-glutamyl transpeptidase (GGT) is produced by up to 31% of strains of Campylobacter jejuni isolates. C. jejuni GGT is close to Helicobacter pylori GGT suggesting a conserved activity but unlike the latter, C. jejuni GGT has not been studied
Hui Zheng et al.
Molecules (Basel, Switzerland), 18(11), 13425-13433 (2013-11-02)
A novel chemoenzymatic one-pot multicomponent synthesis of thiazole derivatives was developed. A series of thiazole derivatives were synthesized with high yields up to 94% under mild enzyme-catalyzed conditions. The blank and control experiments reveal that trypsin from porcine pancreas (PPT)
Articoli
Evaluation of Recombinant, Chemically Treated Trypsin in Proteomics and Protein Characterization Assays
Get better detection and quantification of proteases with this high-sensitivity red protease detection assay.
Pretreatment with Mucinase StcE increases glycopeptide identification from mucin samples, enhancing sample preparation efficiency for glycopeptide analysis.
The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
Protocolli
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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What can I use to solubilize this Product T6567, Trypsin from porcine pancreas?
1 risposta-
Utile?
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How can we digest protein using Product T6567, Trypsin from porcine pancreas?
1 risposta-
As per Sigma R and D the information is as follows: In order to efficiently digest a protein with trypsin, it must be denatured and the disulfide bonds modified by reduction and alkylation, or at least reduced. Many intact proteins are highly resistant to digestion with trypsin. If you do not want to reduce and alkylate, you can then just boil the protein with 5 mM DTT or 20 mM 2ME for 10 minutes, and then quickly cool on ice to denature the protein. This may result in a precipitate, but the trypsin will still digest the protein and it will clear within an hour or two. You can also dissolve the protein in 6 M guanidine-HCl or 8 M urea. Reduce and alkylate using the PROT-RA kit or other suitable method. Then they would have to dilute the solution to less than 2 M of either denaturant and then add the trypsin. This is the method that we use routinely in the lab.
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product T6567, Trypsin from porcine pancreas, TPCK treated?
1 risposta-
The product has been treated with TPCK to remove chymotryptic activity, further purified through affinity chromatography, and lyophilized, resulting in convenient use and highly specific cleavage. This information is on the product page under application.
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What is the package size of Product T6567, Trypsin from porcine pancreas?
1 risposta-
Each vial content 20 ug of the product.
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How can we get Trypsin sequence information for Product T6567, Trypsin from porcine pancreas?
1 risposta-
If you are looking for trypsin sequence information, you have to go to the NCBI Protein Data Bank.
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