SRP6158

EPO human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinonimo/i: EP, EPO-alpha, Epoetin, Erythropoietin-alpha, MGC138142
• Numero CAS: 11096-26-7
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized
• PM: 36 kDa (monomer, glycosylated)
• Confezionamento: pkg of 10 μg
• tecniche: cell culture | mammalian: suitable
• N° accesso NCBI: P01588
• N° accesso UniProt: P01588
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... EPO(2056)

Descrizione

Descrizione generale

The gene EPO (erythropoietin) is mapped to human chromosome 7q22.[1] It is a glycoprotein hormone. EPO is generated in fetal liver and adult kidney. However, it can also be secreted by other organs, such as the heart, brain and lungs.[2] EPO is a member of the EPO/TPO (thrombopoietin) family and is found in the plasma.[3]

Applicazioni

• EPO (erythropoietin) has been used as a component of the culture medium for cardiac stem cells.[4]
• In cultured cerebellar granule cells and hippocampal neurons, it has been used to study effect of EPO on glutamate release.[5]
• It has also been used as a component of endothelial differentiation medium.[6]

Azioni biochim/fisiol

EPO (erythropoietin) regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.[3] It interacts with EPO receptor and is responsible for the cellular responses. EPO is expressed in many cancers, including breast cancer, renal cancer, gastric cancer, hepatocellular carcinoma and central nervous system tumors. In addition, it is a hypoxia responsive cytokine and a pro-angiogenic factor.[2] This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.[7] It can protect from brain damages due to ischemia and enhances memory as well as cognitive ability in humans suffering from vascular dementia.[7]

Stato fisico

Lyophilized from a PBS solution.

Nota sulla preparazione

Centrifuge the vial prior to opening.

Ricostituzione

Reconstitute in sterile PBS containing 0.1% endotoxin-free, recombinant human serum albumin.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable