SAE0092
Mutanolisina
free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)
Sinonimo/i:
Mutanolysin
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About This Item
Prodotti consigliati
Origine biologica
Streptomyces sp. (Streptomyces globisporus ATCC 21553)
Forma fisica
lyophilized powder
Attività specifica
≥4000 units/mg protein (biuret)
PM
23 kDa
~18.5 kDa
Caratteristiche
DNA free
Concentrazione
≥40% protein
tecniche
nucleic acid detection: suitable
Compatibilità
suitable for cell lysis
applicazioni
life science and biopharma
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Categorie correlate
Applicazioni
Fornisce una delicata lisi cellulare per l′isolamento di biomolecole facilmente degradabili e di RNA dai batteri. È stata usata nella formazione di sferoplasti per l′isolamento del DNA.
Purified Mutanolysin SAE0092 undergoes strict quality control testing to ensure the absence of detectable levels of contaminating DNA using 35 cycles PCR amplification of 16S and 18S rDNA using universal primer sets.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Mutanolysin is a muralytic enzyme, suitable for usage in isolation of nucleic acids, from Gram-positive bacteria (e.g. Listeria, Lactobacillus, Lactococcus),(8) and also from other bacteria that are difficult to lyse with lysozyme only.
Azioni biochim/fisiol
La mutanolisina è una N-acetilmuramidasi. Come il lisozima, è un enzima muralitico che scinde il legame β-N-acetilmuramil-(1→4)-N-acetilglucosamina del polimero peptidoglicano-polisaccaride della parete cellulare batterica. Le frazioni carbossi-terminali sono coinvolte nel riconoscimento e nel legame dei polimeri unici della parete cellulare. La mutanolisina esegue la lisi di Listeria e di altri batteri gram positivi come Lactobacillus e Lactococcus.
Caratteristiche e vantaggi
Suitable for difficult-to-lyse samples.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Microbial DNA free, minimizing DNA contamination from reagents in workflow.
Suitable for the isolation of nucleic acids from Gram-positive bacteria, including Listeria, Lactobacillus, and Lactococcus.
Definizione di unità
One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.
Stato fisico
Lyophilized powder containing Ficoll® and sodium succinate buffer salts
Note legali
Ficoll is a registered trademark of Cytiva
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I clienti hanno visto anche
Stacey M Broomall et al.
Applied and environmental microbiology, 82(2), 596-607 (2015-11-15)
Effective microbial forensic analysis of materials used in a potential biological attack requires robust methods of morphological and genetic characterization of the attack materials in order to enable the attribution of the materials to potential sources and to exclude other
G B Calandra et al.
Infection and immunity, 28(3), 1033-1037 (1980-06-01)
Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls
Jennifer A Taylor et al.
Current protocols, 1(4), e80-e80 (2021-04-13)
The bacterial cell wall, composed of peptidoglycan (PG), provides structural integrity for the cell and is responsible for cell shape in most bacteria. Here we present tools to study the cell wall using a clickable PG-specific sugar, 2-alkyne muramic acid
Anna Cäcilia Ingham et al.
Applied and environmental microbiology, 87(18), e0122521-e0122521 (2021-07-01)
Drivers of pig trucks constitute a potential route of human transmission of livestock-associated methicillin-resistant Staphylococcus aureus clonal complex 398 (LA-MRSA CC398). In this study, we determined MRSA prevalence in pig truck drivers (n = 47) and monitored the nasal microbiota of 9
K Yokogawa et al.
Antimicrobial agents and chemotherapy, 6(2), 156-165 (1974-08-01)
Mutanolysin partially purified from the culture filtrate of Streptomyces globisporus 1829 consists of two main lytic enzymes with an isoelectric point near pH 8.5 and 10, respectively, and proteolytic enzyme is associated with the latter lytic enzyme. Mutanolysin exhibited maximal
Articoli
Enzymatic cell lysis and protoplast prep processes demystified, revealing complex enzymatic reactions during sample preparation.
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