QPBCA

QuantiPro^™ BCA Assay Kit

for 0.5-30 μg/ml protein

• Sinonimo/i: BCA Assay, Protein determination by BCA, bicinchoninic acid reagent
• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Applicazioni

In addition to protein determination and quantification in solution,[1][2][3] the QuantiPro^™ BCA Assay Kit has other applications, including determination of protein covalently bound to agarose supports and protein adsorbed to multiwell plates. It has also been applied to the determination of functional groups such as sulfhydryl, N-hydroxysuccinimido carboxylate, aldehyde and hydrazide on a variety of solid supports.

The kit can be used to measure very dilute protein concentrations in very small sample volumes. Accurately measures protein concentrations from 0.5 to 30 μg/mL in tube assays and 1 to 20 μg/mL in 96- or 384-well plate assays.

Caratteristiche e vantaggi

• Accurate across a broad range of protein concentrations
• High sensitivity; linear response form 0.5 to 30 μg/ml of protein
• Stable color complex
• Reduced susceptibility to detergents

Principio

Based on the alkaline reduction of Cu²⁺ to Cu¹⁺ by proteins, and the formation of a bicinchoninic acid:Cu¹⁺ complex having an absorbance maximum at 562 nm.

Stoccaggio e stabilità

Store Reagents QA, QB, and QC at room temperature. Reagents QA and QB can be mixed together without Reagent QC (4% Copper(II) Sulfate Pentahydrate Solution 12 mL) and stored in a closed container at room temperature for a week. If this kit is received or stored cold, a precipitate may form in Reagent QA or Reagent QB. To dissolve the precipitate, warm the solution slowly to room temperature while mixing. The solutions are now suitable for use.

Note legali

QuantiPro is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

• Copper(II) sulfate solution, 4 % (w/v) (prepared from copper (II) sulfate pentahydrate) 12 mL

I componenti del kit sono disponibili anche separatamente

• P0914 Protein Standard Solution: 1.0 mg/ml bovine serum albumin in 0.15 M NaCl with 0.05% sodium azide (flame-sealed glass ampules) 10 x 1

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H290,H315,H318,H410
• Consigli di prudenza: P234 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials