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TP0100

Sigma-Aldrich

Total Protein Kit, Micro

Sinonimo/i:

Bradford

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Descrizione

wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L

Parametri

18-26 °C temperature
50 μL sample volume

Temperatura di conservazione

2-8°C

Descrizione generale

Protein determination is one of the most common operations performed in biochemical research. Total protein kit, micro provides a simple, rapid, and highly sensitive method requiring only 50 μL or less sample.

Applicazioni

Total protein kit, micro has been used to determine:
  • total protein in the bacterial homogenate by Bradford method
  • total protein content by the Bradford microassay from the tissue extracts
  • serum total protein
  • soluble protein content by the Bradford method from leaves

Azioni biochim/fisiol

Total protein kit, micro can be used to determine the concentration of proteins in solution.

Compatibilità

Suitable for protein determination for samples of up to 0.5 mg/ml.

Principio

Brilliant blue G (Coomassie blue) reacts with protein in an acid-alcohol medium to form a blue-colored protein dye complex. The color, measured at 595 nm, is proportional to the protein concentration.

Metodo analitico

Colorimetric, Endpoint

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • Protein Standard 5 mL

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1

Organi bersaglio

Eyes,Central nervous system

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

132.8 °F

Punto d’infiammabilità (°C)

56 °C

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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