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TP0300

Sigma-Aldrich

Total Protein Kit, Micro Lowry, Peterson′s Modification

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

impiego

sufficient for ~200 assays

Descrizione generale

This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.

Applicazioni

Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.

Caratteristiche e vantaggi

  • Complete kit for protein assays
  • Most widely cited protein assay
  • Less protein to protein variation than other assays
  • Contains reagents that can remove interfering substances

Principio

An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.

Avvertenze

Danger

Indicazioni di pericolo

H290,H302,H314,H335,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Dalia A Elgamal et al.
Molecular and cellular endocrinology, 503, 110700-110700 (2020-01-07)
Genetic and epigenetic factors contribute equally to the pathogenesis of type 1 diabetes mellitus. Sodium butyrate (NaB) has been reported to improve glucose homeostasis by modulation of the p38/ERK MAPK pathway. This work aims to evaluate the effect of NaB
Chiara Bernardini et al.
BMC complementary and alternative medicine, 18(1), 194-194 (2018-06-27)
It is widely accepted the key role of endothelium in the onset of many chronic and acute vascular and cardiovascular diseases. In the last decade, traditional compounds utilized in "folk medicine" were considered with increasing interest to discover new bioactive
Protective effect of carbon monoxide pre-conditioning on LPS-induced endothelial cell stress
Bernardini C, et al.
Cell Stress & Chaperones, 15(2), 219-224 (2010)
Expression of alpha-gustducin and alpha-transducin, G proteins coupled with taste receptors, in boar sperm
Spinaci M, et al.
Theriogenology, 82(1), 144-151 (2014)
Chiara Bernardini et al.
Journal of inflammation (London, England), 9(1), 47-47 (2012-11-28)
It is well known that nitric oxide (NO) is generated by a family of constitutively (nNOS and eNOS) or inducibly (iNOS) expressed enzymes and takes part in different aspects of the inflammatory response; nevertheless, its effective role in the pathogenesis
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Protocolli

Protein Determination by the Warburg-Christian Method

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Contenuto correlato

Tools for Protein Quantitation

Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.

Quantificazione delle proteine

Metodi di quantificazione delle proteine, reagenti e tecnologie di analisi immunochimica per misurare con precisione la concentrazione delle proteine in una varietà di campioni.

Protein Quantitation

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

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