B6916
Sinonimo/i:
Saggio delle proteine leganti il colorante Coomassie, reagente colorante le proteine
| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
500 mL | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 160,00 € |
1 of 1
Questo articolo | |||
|---|---|---|---|
| description for 0.1-1.4 mg/ml protein | description - | description - | description - |
| form solution | form liquid | form - | form - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level - |
Warning
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 2
Eyes,Central nervous system
Not applicable
Not applicable
12 - Non Combustible Liquids
Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.
Scegli una delle versioni più recenti:
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
For the Standard 3.1 mL Assay and 96 Well Plate Assay, the protein standards should be prepared in the range of 0.1 - 1.4 mg/mL. This is not the final concentration of the protein after being diluted in the assay.
For the full protocol, please refer to the document found here:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/165/479/b6916bul-ms.pdf
Utile?
Yes, phenol red at concentrations above 0.5 mg/mL will cause interference. Concentrations below 0.5 mg/mL are expected to be compatible.
Utile?
The color of this product can range from Faint Yellow-Brown to Light Brown. The exact result will be listed on the lot specific Certificate of Analysis. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under ‘Certificate of Analysis’: https://www.sigmaaldrich.com/product/sigma/b6916#product-documentation
Utile?
A compatibility chart is listed in the bulletin. This chart indicates that this reagent is compatible with concentrations of SDS up to 0.125%.
Utile?
The Bradford method has a lower limit of detection of 20 ug/mL. The Lowry method has a lower limit of 10 ug/mL. Concentration of the sample may be necessary.
See the links below for additional helpful information:
Protein Quantitation Methods-
https://www.sigmaaldrich.com/applications/protein-biology/protein-quantitation
Amicon Centrifugal Filters-
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-concentration-and-buffer-exchange/amicon-ultra-centrifugal-filters
Centricon Centrifugal Filters-
https://www.sigmaaldrich.com/substance/centriconplus70centrifugalfilter1234598765
Utile?
Albumins are readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate. The solution stability of BSA is very good (especially if the solutions are stored as frozen aliquots). In fact, albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat. When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling. At somewhat lower temperatures aggregation is also expected to occur, but at relatively slower rates.
Please see the product data sheet which describes the solution stability of BSA:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/351/531/a8412pis.pdf
Utile?
Is the BSA standard supplied with Product B6916, Bradford Reagent?
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.
Utile?
When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?
he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.
Utile?
Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples.
Utile?
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
