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PKH26PCL

Sigma-Aldrich

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

Distributed for Phanos Technologies

Sinonimo/i:

Phagocytic cell label

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1 KIT
1.670,00 €

1.670,00 €


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Cambia visualizzazione
1 KIT
1.670,00 €

About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

1.670,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Applicazioni

This kit is for phagocytic cell labeling. It is used to selectively label cells with phagocytic capabilities such as monocytes, macrophages or neutrophils.

Principio

The labeling occurs through the formation of dye aggregates or particulates. The aggregate formation significantly inhibits the uptake of dye by non-phagocytic cells, such as lymphocytes, but facilitates dye uptake by phagocytic cells. Labeled cells appear patchy or spotted because the dye is localized in phagocytic compartments of the cells. The dye appears to be resistant to metabolic attack and has been found to remain with the cells for at least 21 days in vivo.
Labeling of phagocytic cells by this methodology may be conducted either in vitro or in vivo. Intraperitoneal or intravenous injections of the PKH26 labeling solution will successfully label phagocytic cells in vivo, while cells of interest which have been isolated may be stained using in vitro labeling methods.

Linkage

For additional technical details on PKH and CellVue® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here.

Note legali

CellVue is a registered trademark of Phanos Technologies

Solo come componenti del kit

N° Catalogo
Descrizione

  • Diluent B 6 x 10

  • PKH26 cell linker in ethanol .5 mL

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

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Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

57.2 °F - closed cup

Punto d’infiammabilità (°C)

14.0 °C - closed cup


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K Tabata et al.
Gene therapy, 18(10), 969-978 (2011-04-23)
We previously identified the mouse and human Glipr1 and GLIPR1/RTVP-1 genes, respectively, as direct p53 targets with proapoptotic activities in various cancer cell lines, including prostate cancer (PCa). Intratumoral injection of an adenoviral vector capable of efficient transduction and expression
Hyung-Syup Kim et al.
Journal of Korean Neurosurgical Society, 44(4), 249-255 (2008-12-20)
In Moyamoya disease, the primary goal of treatment is to improve collateral circulation through angiogenesis. In the present study, we obtained and sub-cultured bone marrow stromal cells (BMSCs) from rats without a cell-mediated immune response. Then, we injected the labeled
Jaime Murphy et al.
American journal of respiratory cell and molecular biology, 38(4), 380-385 (2008-01-15)
To further examine the half-life of alveolar macrophages, chimeric CD 45.2 mice were generated through bone marrow transplantation of donor CD 45.1 cells. Before administration of donor cells, recipient mice were divided into two cohorts: the first cohort received total
Jeffrey H Jennings et al.
American journal of respiratory cell and molecular biology, 32(2), 108-117 (2004-11-26)
Apoptotic cells must be cleared to resolve inflammation, but few resident alveolar macrophages (AMo) from normal lungs ingest apoptotic cells. We examined how Mo ingestion of apoptotic cells is altered during immune inflammation induced by intratracheal challenge of primed C57BL/6
Hiroshi Kanno et al.
International journal of molecular sciences, 14(5), 9604-9617 (2013-05-07)
A specialized population of cells residing in the hair follicle is quiescent but shows pluripotency for differentiating into epithelial-mesenchymal lineage cells. Therefore, such cells are hoped to be useful as implantable donor cells for regenerative therapy. Recently, it was reported

Articoli

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Domande

  1. Is this kit suitable for label circulatory fibrocyte isolated from rat PBMC?

    1 risposta
    1. The PKH cell linker kits have not been specifically tested for the detection of circulatory fibrocytes. However, there are several publications that indicate suitability. Please see the link below to review one such reference:
      https://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13080

      Utile?

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