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MAK099

Sigma-Aldrich

Glutamate Dehydrogenase (GDH) Activity Assay Kit

sufficient for 100 colorimetric tests

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 100 colorimetric tests

Metodo di rivelazione

colorimetric

Malattie correlate

cancer; gastrointestinal diseases

Temperatura di conservazione

−20°C

Informazioni sul gene

Descrizione generale

Glutamate Dehydrogenase (GDH) is a mitochondrial enzyme that catalyzes the reversible oxidative deamination of glutamate to α-ketoglutarate and serves as a key link between anabolic and catabolic pathways. In mammals, GDH is subject to allosteric regulation and has high activity in liver, kidney, brain, and pancreas.[1] GDH activity in serum can be used to differentiate between liver diseases due to liver inflammation, which do not show elevated serum GDH activity, and diseases that result in hepatocyte necrosis, which results in elevated serum GDH.[2]

Applicazioni

Glutamate Dehydrogenase (GDH) Activity Assay Kit has been used to determine the enzymatic activity of glutamate dehydrogenase.[3][4]

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

Suitable for detecting glutamate dehydrogenase (GDH) activity in serum or tissue and cell extracts.

Principio

GDH activity is determined by a coupled enzyme assay in which glutamate is consumed by GDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the GDH activity present. One unit of GDH is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 7.6 at 37 °C.

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Codice della classe di stoccaggio

10 - Combustible liquids


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Domande

  1. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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