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10197734001

Roche

Glutamate Dehydrogenase (GlDH)

from beef liver

Sinonimo/i:

DNA marker

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About This Item

Classificazione EC (Enzyme Commission):
1.4.1.3 (BRENDA, IUBMB)
Codice UNSPSC:
12352204

Origine biologica

bovine liver

Livello qualitativo

100

Forma fisica

lyophilized

Attività specifica

10 U/mg
~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)

Confezionamento

pkg of 3,000 U

Produttore/marchio commerciale

Roche

Concentrazione

≥10-20 % (w/w)

tecniche

activity assay: suitable

Colore

white

pH ottimale

8

Solubilità

water: 20 mg/mL, colorless

Compatibilità

suitable for UV spectrophotometry and general use

N° accesso UniProt

P00366

applicazioni

life science and biopharma

Attività estranea

ADH <0.00500%
LDH <0.00500%
MDH <0.00500%

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Informazioni sul gene

bovine ... GLUD1(281785)

Categorie correlate

Descrizione generale

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.
GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)+ oxidoreductase is involved in deamination.

Applicazioni

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Qualità

Contaminants: <0.005% ADH, LDH, and MDH, each

Sequenza

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

Altre note

For life science research only. Not for use in diagnostic procedures.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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&#945;-Ketoglutaric acid disodium salt dihydrate &#8805;98.0% (dried material, NT)

Sigma-Aldrich

75892

α-Ketoglutaric acid disodium salt dihydrate

Ammonia Assay Kit sufficient for 100&#160;assays

Sigma-Aldrich

AA0100

Ammonia Assay Kit

T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
P J O'Brien et al.
Laboratory animals, 36(3), 313-321 (2002-07-30)
In a recent study in rats, alanine aminotransferase (ALT), the preferred plasma biomarker of hepatocellular injury in rats, was ineffective at detecting marked hepatic necrosis produced by acetaminophen (Human and Experimental Toxicology 19, 277-83, 2000). In contrast, glutamate dehydrogenase (GLDH)
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
Po Chen et al.
Fly, 14(1-4), 3-9 (2020-07-04)
Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous species (e.g. mouse, rat, zebrafish, chicken) including humans and Drosophila encode two broadly expressed copies

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