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L2157

Sigma-Aldrich

Laccase from Rhus vernicifera

greener alternative

crude acetone powder, ≥50 units/mg solid

Sinonimo/i:

Benzenediol:oxygen oxidoreductase

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10000 UNITS
184,00 €

184,00 €


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10000 UNITS
184,00 €

About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

184,00 €


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Stato

crude acetone powder

Livello qualitativo

Attività specifica

≥50 units/mg solid

Caratteristiche più verdi

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

Temperatura di conservazione

−20°C

Stringa SMILE

[n]1(c(c(cc1C)C=O)C)CC

InChI

1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3
NWDZDFOKSUDVJV-UHFFFAOYSA-N

Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biofuel cell and cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research.

Definizione di unità

One unit will produce a ΔA530 of 0.001 per min at pH 6.5 at 30 °C in a 3 mL reaction volume using syringaldazine as substrate.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Protocolli

Laccase, a biocatalyst, oxidizes aromatic compounds with molecular oxygen reduction, making it useful in various applications.

Domande

1–6 di 6 domande  
  1. What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?

    1 risposta
    1. Our own assay is performed at 30°C.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.

      Utile?

  2. My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?

    1 risposta
    1. Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris.  A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.

      Utile?

  3. What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?

    1 risposta
    1. Our own assay is performed at pH 6.5.  One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.

      Utile?

  4. How stable are solutions of Product L2157, Laccase from Rhus vernicifera?

    1 risposta
    1. Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.

      Utile?

  5. What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?

    1 risposta
    1. Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.

      Utile?

  6. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

Recensioni

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