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GE28-9893-36

Sigma-Aldrich

HiLoad® 26/600 Superdex® 200 pg

Cytiva 28-9893-36, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

Sinonimo/i:

HiLoad Superdex 200

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

Materiali

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

PM

10000-600000 Da (globular proteins)

Confezionamento

pkg of 1 ea

Produttore/marchio commerciale

Cytiva 28-9893-36

Parametri

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa

Volume del letto

320 mL

Lungh. × diam. int. colonna

60 cm × 26 mm

Matrice

agarose-dextran composite
dextran-agarose particle platform
polymeric

Gruppo funzionale matrice

dextran-agarose phase
dextran-agarose phase

Dimens. media delle particelle

34 μm

cleaning

1-14

working range

3-12

Tecnica di separazione

size exclusion (SEC)

Categorie correlate

Descrizione generale

HiLoad® 26/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice highly suitable for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Caratteristiche e vantaggi

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Compatibilità

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Note legali

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocolli

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Contenuto correlato

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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