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GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Sinonimo/i:

16/600 Superdex 200, HiLoad Superdex 200

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2.850,00 €

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

2.850,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Materiali

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

PM

10000-600000 Da (globular proteins)

Confezionamento

pkg of 1 ea

Produttore/marchio commerciale

Cytiva 28-9893-35

Parametri

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

Volume del letto

120 mL

Lungh. × diam. int. colonna

60 cm × 16 mm

Matrice

agarose dextran composite
dextran-agarose particle platform
polymeric

Gruppo funzionale matrice

dextran-agarose phase

Dimens. media delle particelle

34 μm

cleaning

1-14

Intervallo di esercizio

3-12

Tecnica di separazione

size exclusion (SEC)

Descrizione generale

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Caratteristiche e vantaggi

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Compatibilità

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Protocolli

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Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows the purification strategy of capture, intermediate purification, and polishing (CIPP) in affinity chromatography using Cytiva products.

This page shows how to refold proteins using gel filtration and products from Cytiva.

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Contenuto correlato

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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