Sigma recommends to dissolve the product with 75 mM phosphate buffer, pH 7.2, containing 25% (v/v) glycerol to a concentration of 2-5 units per mL. Keep the solution in an ice bath while using and store solution frozen. This solution may be stored frozen and thawed several times with only slight loss of activity.
G7509
GABase from Pseudomonas fluorescens
lyophilized powder, Protein ≥30 % by biuret
Sinonimo/i:
GABase Enzyme, GABase from Pseudomonas, Pseudomonas GABase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
10 UNITS
525,00 €
25 UNITS
1.150,00 €
Scegli un formato
Cambia visualizzazione
10 UNITS
525,00 €
25 UNITS
1.150,00 €
About This Item
Prodotti consigliati
Origine biologica
Pseudomonas fluorescens
Livello qualitativo
Saggio
≥30 % (biuret)
Stato
lyophilized powder
Attività specifica
≥0.5 units/mg protein
Composizione
Protein, ≥30% biuret
tecniche
inhibition assay: suitable
Temperatura di conservazione
−20°C
Descrizione generale
Applicazioni
Azioni biochim/fisiol
GABase catalyzes the conversion of γ-aminobutyric acid (GABA) to succinic acid in the central nervous system. It is used to measure GABA levels in various biological samples. p-Hydroxybenzaldehyde (HBA) is known to be a potential inhibitor on GABase.[3]
Definizione di unità
One unit will convert 1.0 μmole of γ-aminobutyric acid (GABA) to succinic semialdehyde and then to succinate per min with a stoichiometric reduction of 1.0 μmole of NADP+ at pH 8.6 at 25 °C.
Stato fisico
Partially purified lyophilized powder containing buffer salts and stabilizer
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
GABase and glutaminase inhibitory activities of herbal extracts and acylated flavonol monoglycosides isolated from the leaves of Laurus nobilis L
Shimada A, et al.
Zeitschrift fur Naturforschung. C, Journal of biosciences, 78(9-10), 377-381 (2023)
A Vidal-Cros et al.
The Biochemical journal, 229(3), 675-678 (1985-08-01)
L-threo-3-Fluoroglutamate and L-erythro-3-fluoroglutamate were tested with glutamate decarboxylase from Escherichia coli. Both isomers were substrates: the threo isomer was decarboxylated into optically active 4-amino-3-fluorobutyrate, whereas the erythro isomer lost the fluorine atom during the reaction, yielding succinic semialdehyde after hydrolysis
Mingu Gordon Park et al.
Journal of enzyme inhibition and medicinal chemistry, 36(1), 2016-2024 (2021-09-14)
Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification have not been established. Thus, biochemical and drug
Structural and Mechanistic Basis for the Inactivation of Human Ornithine Aminotransferase by (3 S,4 S)-3-Amino-4-fluorocyclopentenecarboxylic Acid
Shen S, et al.
Molecules (Basel), 28(3) (2023)
Nicolaie Moldovan et al.
Sensors and actuators. B, Chemical, 337 (2022-05-24)
Glutamate (GLU) and gamma-aminobutyric acid (GABA) are neurotransmitters (NTs) with an essential role in signal transmission in the brain. Brain disorders, such as epilepsy, Alzheimer's and Parkinson's diseases, and traumatic brain injury can be linked to imbalances in the GLU-GABA
-
How do I solubilize Product No. G7509, GABase from Pseudomonas fluorescens?
1 risposta-
Utile?
-
-
What is the shelf life of the Product No.G7509, GABase from Pseudomonas fluorescens?
1 risposta-
Sigma recommends retesting the product 3 years after its release date. The retest date will appear on the lot specific Certificate of Analysis for the product.
Utile?
-
-
Are there any literature references that cite use of the Product No. G7509, GABase from Pseudomonas fluorescens?
1 risposta-
The product is cited in the reference Sulaimana, Saba A.J., et al., J of Enz Inhib and Med Chem. 18(4), 297-301 (2003).
Utile?
-
-
What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
