The K12 strain information can be found on the UniProt website under the following entry: P0A9C5.
G1270
L-Glutamine Synthetase from Escherichia coli
lyophilized powder, 400-2,000 units/mg protein
Sinonimo/i:
L-Glutamate:ammonia ligase (ADP-forming)
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
100 UNITS
542,00 €
Scegli un formato
Cambia visualizzazione
100 UNITS
542,00 €
About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.26
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
Attività specifica
400-2,000 units/mg protein
Purificato mediante
affinity chromatography
contiene
dithioerythritol as preservative
Composizione
Protein, ~5% Lowry
Solubilità
H2O: soluble 0.95-1.05 mg/mL, clear to hazy
N° accesso UniProt
Attività estranea
ATPase <0.2%
Temperatura di conservazione
−20°C
Informazioni sul gene
Escherichia coli K12 ... glnA(948370)
Descrizione generale
L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.[1]
Applicazioni
L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli[2] and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.[3]
L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.[4]
Azioni biochim/fisiol
L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine[5]. It is a degradative enzyme for glutamic acid.
Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli.[1] GS plays a key role in ammonia assimilation in bacteria.[6] Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.[7]
Degradative enzyme for glutamic acid
Definizione di unità
One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.
Stato fisico
Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Reversible Adenylylation of Glutamine Synthetase Is Dynamically Counterbalanced during Steady-State
Okano H, et al.
Journal of molecular biology, 404(1), 522-536 (2010)
Regulatory role for a novel human thioredoxin peroxidase in NF-kappaB activation
Jin DY, et al.
The Journal of Biological Chemistry, 272(49), 30952-30961 (1997)
Purification of a protease from Escherichia coli with specificity for oxidized glutamine synthetase.
J E Roseman et al.
The Journal of biological chemistry, 262(5), 2101-2110 (1987-02-15)
A soluble Escherichia coli protease has been identified and purified to homogeneity. The protease cleaves glutamine synthetase which has been modified by mixed function oxidation; native glutamine synthetase is not a substrate. Using [14C]glutamine synthetase as a substrate (prepared by
S H Liaw et al.
Protein science : a publication of the Protein Society, 4(11), 2358-2365 (1995-11-01)
Glutamine synthetase (GS) catalyzes the ATP-dependent condensation of ammonia and glutamate to yield glutamine, ADP, and inorganic phosphate in the presence of divalent cations. Bacterial GS is an enzyme of 12 identical subunits, arranged in two rings of 6, with
E Rodriguez et al.
Plant physiology and biochemistry : PPB, 62, 19-22 (2012-11-24)
Lead (Pb) is a toxic element, but its putative mutagenic effects in plant cells, using molecular markers, remain to unveil. To evaluate if Pb induces mutagenicity, Pisum sativum L. seedlings were exposed to Pb(2+) (up to 2000 mg L(-1)) for 28 days
-
From which E. coli strain is your product G1270 L-Glutamine Synthetase from Escherichia coli isolated?
1 risposta-
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
