This product is a stabilized solution in Tris buffer, pH 7.3.
G0774
Glycerokinase from Bacillus stearothermophilus
buffered aqueous solution, ≥75 units/mg protein (biuret)
Sinonimo/i:
ATP:glycerol 3-phosphotransferase, Glycerol Kinase
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500 UNITS
403,00 €
403,00 €
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500 UNITS
403,00 €
About This Item
403,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Origine biologica
Bacillus sp. (Bacillus steaarothermophilus)
Livello qualitativo
Saggio
≥5.0 mg protein/mL (biuret)
Stato
buffered aqueous solution
Attività specifica
≥75 units/mg protein (biuret)
Condizioni di stoccaggio
dry at room temperature
Colore
beige
applicazioni
life science and biopharma
Temperatura di conservazione
2-8°C
Informazioni sul gene
Bacillus sp. ... glpK(89613566)
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Descrizione generale
Research area: Cell Signaling
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Glycerol kinase is encoded by the GK gene on chromosome Xp21.2. Glycerol kinase is predominantly active in the liver.
Applicazioni
Glycerokinase from Bacillus stearothermophilus has been used in steady-state kinetics studies.[1]
Azioni biochim/fisiol
Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol.[2][3] It is also subject to feedback regulation by fructose-1,6-bisphosphate.[4]Oxidized glycerol-3-phosphate results in dihydroxyacetone phosphate which enters either glycolysis or gluconeogenesis. Elevated glycerol is observed in glycerol kinase deficiency leading to pseudohypertriglyceridemia.
Definizione di unità
One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25 °C in a coupled system with PK/LDH.
Stato fisico
Stabilized solution in Tris buffer, pH 7.3
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Fructose-1 ,6-diphosphate is a feedback inhibitor of the catabolic enzyme, glycerol kinase, in Escherichia coli. A mutant was isolated which produced a desensitized enzyme. Glucose was no longer as effective in preventing the utilization of exogenous glycerol by cells which
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Hello What is the composition of the "Buffered Aqueous Solution" for the G0774--Sigma-Aldrich. Glycerokinase from Bacillus stearothermophilus. This information is important and necessary for our work. Thanks
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