When considering your question, the initial response was that Fast Red TR is typically used for light microscopy. I consulted Conn's Biological Stains, 10th Edition from the Biological Stain Commission and found a reference for its use in fluorescence microscopy in an article by Lizard, G et al (1997). The article is titled "Detection of human papillomavirus DNA in genital lesion by enzymatic in situ hybridization with fast red and laser confocal microscopy" and was published in the Histochemical Journal (29: 544-554).
F4648
Fast Red TR/Naphthol AS-MX
SIGMAFAST™, fluorogenic, tablet
Sinonimo/i:
4-Chloro-2-methylbenzenediazonium / 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate tablets
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5 SETS
45,50 €
50 SETS
231,00 €
45,50 €
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Cambia visualizzazione
5 SETS
45,50 €
50 SETS
231,00 €
About This Item
45,50 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Nome del prodotto
SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets, tablet, To prepare 1 mL
Livello qualitativo
Stato
tablet
Solubilità
water: 1 tablet/mL, clear to hazy, colorless to faintly yellow
Temperatura di conservazione
−20°C
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Categorie correlate
Applicazioni
SIGMAFAST Fast Red TR/Naphthol AS-MX Tablets has been used:
- in alkaline phosphatase staining in organ sections[1]
- for staining chondrogenic spheroids in immunohistochemistry studies[2]
- for the detection of hybridization signals in in situ mRNA hybridization studies of human palatine tonsils sections[3]
Developed for use as a precipitating substrate for the detection of alkaline phosphatase activity in immunochemistry. Primary uses are in immunohistology, immunoblotting, or dot blotting. SIGMAFAST™ Fast Red TR/Naphthol AS-MX tablets contain 0.6 mM levamisole to block endogenous alkaline phosphatase activity. SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets require no additional steps or buffers to prepare an active substrate solution.
Ricostituzione
Each tablet dissolved in 1 mL deionized water yields a ready-to-use buffered solution.
Note legali
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1C
Codice della classe di stoccaggio
8A - Combustible corrosive hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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Could product F4648 be used with a 543 laser system?
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