E1131
Exonuclease III from Escherichia coli BE25 /psGR3
buffered aqueous glycerol solution
Autenticati per visualizzare i prezzi organizzativi e contrattuali.
Informazioni su questo articolo
Numero CAS:
UNSPSC Code:
12352204
EC Number:
232-912-1
MDL number:
grade
Molecular Biology
form
buffered aqueous glycerol solution
mol wt
28 kDa
UniProt accession no.
storage temp.
−20°C
Gene Information
Escherichia coli K12 ... xthA(946254)
General description
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
Application
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
Other Notes
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.