CS0390
Mitochondria Staining Kit
1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)
Sinonimo/i:
JC-1 dye
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Codice UNSPSC:
12352207
NACRES:
NA.32
Prodotti consigliati
Livello qualitativo
impiego
kit sufficient for 200 tests (of 1 mL cell suspensions)
kit sufficient for 40 tests (of 5 mL cell suspensions)
Confezionamento
pkg of 1 kit
Condizioni di stoccaggio
dry at room temperature
tecniche
flow cytometry: suitable
protein staining: suitable
Fluorescenza
λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)
applicazioni
cell analysis
detection
Metodo di rivelazione
fluorometric
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.
Applicazioni
The dissipation of the mitochondrial electrochemical potential gradient (Δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.
Caratteristiche e vantaggi
Kit offers:
- A fast, simple, and convenient method for staining and assaying mitochondria intactness
- Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential
- Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation
- The shift in fluorescence is clearly detectable in both green and red channels
- Suitable for adherent cells as well as cells in suspension
- Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells
- The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry
Solo come componenti del kit
N° Catalogo
Descrizione
- DMSO 1 mL
- JC Staining Buffer 5X 120 mL
- JC-1 1 mg
- Valinomycin Ready Made .1 mL
Codice della classe di stoccaggio
10 - Combustible liquids
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