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MITOISO1

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Sinonimo/i:

Mitochondria Purification Kit

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1 KIT
1.180,00 €

1.180,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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1 KIT
1.180,00 €

About This Item

Numero CE:
232-650-8
Codice UNSPSC:
12352200
NACRES:
NA.32

1.180,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Livello qualitativo

200

impiego

sufficient for 10-20 g (animal tissue)
 sufficient for 50 assays (2 mL)

Durata

2 yr

tecniche

fractionation: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Categorie correlate

Applicazioni

Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity[1]
  • for the isolation of mitochondria from glomeruli[2] and heart[3]
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential[4]

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.

Caratteristiche e vantaggi

  • Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
  • Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
  • Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
  • Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDS

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CS0720

Citrate Synthase Assay Kit, 1 kit sufficient for 100 reactions (using a 1 ml cuvette), 1 kit sufficient for 480 reactions (using 96 multiwell plates)

MITOISO2

Mitochondria Isolation Kit, sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

CS0760

Isolated Mitochondria Staining Kit, 1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

MITOISO3

Yeast Mitochondria Isolation Kit, sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells

Pittogrammi

Health hazardExclamation mark
GHS08,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H315,H319,H334,H335

Classi di pericolo

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

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Certificati d'analisi (COA)

Lot/Batch Number
0000284757
0000284757
0000222575
0000222575
0000137714

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Janna R Jackson et al.
The journals of gerontology. Series A, Biological sciences and medical sciences, 66(7), 751-764 (2011-04-02)
This study analyzed the capacity of resveratrol, a naturally occurring polyphenol, to reduce aging-induced oxidative stress and protect against sarcopenia. Middle-aged (18 months) C57/BL6 mice were randomly assigned to receive either a control diet or a diet supplemented with 0.05%
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Articoli

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

Domande

1–8 di 8 domande  

  1. Where is the step to get cytosol?

    1 risposta

    1. The kit is intended for the isolation of intact mitochondria. It was not developed for cytoplasma collection. Nevertheless, reviewing the protocol, it can be assumed that the cytoplasmic fraction should be in the supernatant after the second centrifugation (point 8 of the Preparation of mitochondria from soft tissues protocol). Please keep in mind that this is not tested for suitability for cytoplasmic fraction.

      Please see product MIT1000 Mitochondria/Cytosol Fractionation Kit for efficient isolation of intact mitochondria from cultured cells. This kit can be used for tissue samples as long as the tissue is first homogenized into a single cell suspension. It may be helpful to use the same protocol that is recommended for cells following homogenization of the tissue into a single cell suspension in the supplied Isotonic Mitochondrial Buffer containing protease inhibitors, then follow the kits protocol for cells: https://www.sigmaaldrich.com/product/mm/mit1000.

      Utile?

  2. Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?

    1 risposta

    1. It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.

      Utile?

  3. When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?

    1 risposta

    1. If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

      Utile?

  4. When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?

    1 risposta

    1. Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

      Utile?

  5. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  6. Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?

    1 risposta

    1. The extraction buffers supplied with this kit are only available with the kit.

      Utile?

  7. How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?

    1 risposta

    1. The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.

      Utile?

  8. Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?

    1 risposta

    1. Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.

      Utile?

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