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C4710

Sigma-Aldrich

Monoclonal Anti-Cyclin A antibody produced in mouse

clone CY-A1, ascites fluid

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

CY-A1, monoclonal

PM

antigen 60 kDa

contiene

15 mM sodium azide

Reattività contro le specie

mouse, human, monkey, bovine

tecniche

immunocytochemistry: suitable
microarray: suitable
western blot: 1:1,000 using a mouse NIH 3T3 cell extract

Isotipo

IgG2a

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... CCNA2(890)
mouse ... Ccna2(12428)

Descrizione generale

Cyclin A is a nuclear protein during S phase and it binds both cdk2 and cdc2. The availability of monoclonal antibody reacting specifically with cyclin A enables the subcellular detection and localization of cyclin A and the measurement of relative differences in cyclin A levels as a function of cell cycle phase.

Specificità

The antibody recognizes an epitope present in amino acids 162-433 of bovine cyclin A. No cross-reactivity is detected with other cyclins (cyclin B, D, or E).

Immunogeno

recombinant bovine cyclin A.

Applicazioni

Monoclonal Anti-Cyclin A antibody produced in mouse is suitable for:
  • immunocytochemistry
  • microarray
  • western blotting at a dilution of 1:1,000 using a mouse NIH 3T3 cell extract

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Matthew Hoare et al.
Nature cell biology, 18(9), 979-992 (2016-08-16)
Senescence, a persistent form of cell-cycle arrest, is often associated with a diverse secretome, which provides complex functionality for senescent cells within the tissue microenvironment. We show that oncogene-induced senescence is accompanied by a dynamic fluctuation of NOTCH1 activity, which
Qing-Hua Zhang et al.
The Journal of cell biology, 216(10), 3133-3143 (2017-08-19)
Cyclin A2 is a crucial mitotic Cdk regulatory partner that coordinates entry into mitosis and is then destroyed in prometaphase within minutes of nuclear envelope breakdown. The role of cyclin A2 in female meiosis and its dynamics during the transition
Emma L Hesketh et al.
Cell cycle (Georgetown, Tex.), 14(3), 333-341 (2015-02-07)
The minichromosome maintenance complex (MCM2-7) is the putative DNA helicase in eukaryotes, and essential for DNA replication. By applying serial extractions to mammalian cells synchronized by release from quiescence, we reveal dynamic changes to the sub-nuclear compartmentalization of MCM2 as
J E Dazard et al.
Oncogene, 19(33), 3693-3705 (2000-08-19)
p53 transcription factor is mutated in most skin cell carcinomas and in more than 50% of all human malignancies. One of its transcriptional targets is MDM2, which in turn down-regulates p53. The role of the p53/MDM2 regulatory loop upon genotoxic
Chunyan Weng et al.
International journal of oncology, 42(1), 327-337 (2012-11-09)
Androgen deprivation therapy of prostate cancer with estrogens shows significant cardiovascular side-effects. To develop effective prostate cancer therapeutic agent(s) with minimal cardiovascular side-effects, we compared the effects of various estrogen receptor (ER) ligands on the modulation of dihydrotestosterone (DHT) actions

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