C2014

Collagenasi

Name: Collagenasi
Brand: SIGMA

0.2 μm filtered, Type V-S, ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

Sinonimo/i:

Clostridiopeptidasi A

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Informazioni su questo articolo

Numero CAS:

9001-12-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-582-9

MDL number:

MFCD00130830

Numero CE:

3.4.24.3 (BRENDA, IUBMB)

Specific activity:

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

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sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥1 FALGPA units/mg solid, ≥125 CDU/mg solid

mol wt

68-125 kDa

storage temp.

−20°C

Quality Level

200

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Questo articolo	C5894	C9263	C7657
Sigma-Aldrich C2014 Collagenase from Clostridium histolyticum	Sigma-Aldrich C5894 Collagenase from Clostridium histolyticum	Sigma-Aldrich C9263 Collagenase from Clostridium histolyticum	Sigma-Aldrich C7657 Collagenase from Clostridium histolyticum
specific activity ≥1 FALGPA units/mg solid, ≥125 CDU/mg solid	specific activity ≥125 CDU/mg solid, 0.5-5.0 FALGPA units/mg solid	specific activity ≥1 FALGPA units/mg solid, >125 CDU/mg solid	specific activity ≥800 CDU/mg solid, 2-5 FALGPA units/mg solid
form lyophilized powder	form lyophilized powder	form powder	form powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 68-125 kDa	mol wt 68-130 kDa	mol wt 113-114 kDa	mol wt 68-130 kDa
sterility 0.2 μm filtered	sterility non-sterile; 0.2 μm filtered	sterility -	sterility -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

Application

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, lung epithelium and many other tissues. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes.

Biochem/physiol Actions

La collagenasi è attivata da quattro grammi atomo di calcio per mole di enzima. È inibita da etilen-glicol-bis(beta-amminoetil etere) - acido N, N, N′,N′-tetraacetico, beta-mercaptoetanolo, glutatione, acido tioglicolico e 8-idrossichinolina.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa (pH optimum: 6.3-8.8). The enzyme recognizes the sequence -R-Pro-8-X-Gly-Pro-R-, where X is most often a neutral amino acid.

Preparation Note

Prepared from Type V (C9263)

Analysis Note

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

Other Notes

Una unità di digestione del collagene (CDU) libera peptidi da collagene proveniente da tendine d′Achille bovino equivalente in colore ninidrina a 1,0 μmole di leucina in 5 ore a pH 7,4 a 37 °C in presenza di ioni calcio. Una unità di idrolisi FALGPA idrolizza 1,0 μmole di furilacriloil-Leu-Gly-Pro-Ala per minuto a 25 °C. Una unità di proteasi neutra idrolizza caseina e produce colore equivalente a 1,0 μmole di tirosina per 5 ore a pH 7,5 a 37 °C. Una unità di clostripaina idrolizza 1,0 μmole of BAEE per minuto a pH 7,6 a 25 °C in presenza di DTT.

used together

N° Catalogo

Descrizione

Determinazione del prezzo

F5135

N-[3-(2-Furyl)acryloyl]-Leu-Gly-Pro-Ala

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number

0000503750

0000302834

0000137861

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[Dupuytren contracture].

R E Giunta et al.

MMW Fortschritte der Medizin, 153(49-50), 41-43 (2012-02-09)

Skin laceration as a serious adverse sequela of injectable collagenase for Dupuytren contracture.

Geoffrey G Hallock

Plastic and reconstructive surgery, 129(1), 205e-206e (2011-12-22)

Assessment of potential cross-reactivity of human endogenous matrix metalloproteinases with collagenase Clostridium histolyticum antibodies in human sera obtained from patients with Dupuytren's contracture.

Thomas J Edkins et al.

Clinical and vaccine immunology : CVI, 19(4), 562-569 (2012-02-24)

Collagenase Clostridium histolyticum (CCH) contains a fixed ratio of class I (AUX-I) and class II (AUX-II) collagenases and is used as treatment for Dupuytren's contracture. These two Zn-dependent enzymes, produced by the Gram-positive bacterium Clostridium histolyticum, are related functionally to

Determining clinically important changes in range of motion in patients with Dupuytren's Contracture: secondary analysis of the randomized, double-blind, placebo-controlled CORD I study.

Jörg Witthaut et al.

Clinical drug investigation, 31(11), 791-798 (2011-10-05)

Injectable collagenase Clostridium histolyticum is efficacious in correcting Dupuytren's contracture as assessed by changes in the angle of contracture and range of motion (ROM). However, clinically important changes in ROM have not been evaluated in depth. The objective of this

Efficacy of collagenase in patients who did and did not have previous hand surgery for Dupuytren's contracture.

Chris Bainbridge et al.

Journal of plastic surgery and hand surgery, 46(3-4), 177-183 (2012-06-08)

Collagenase Clostridium histolyticum (CCH) is a non-surgical, efficacious therapy for Dupuytren's contracture (DC). This study evaluated the efficacy and safety of CCH in patients with previous DC surgery. Data from 12 CCH clinical trials were pooled. At screening, patients provided

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Protocolli

Enzymatic Assay of Collagenase (EC 3.4.24.3) using FALGPA (N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala)

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

Contenuto correlato

Product Information Sheet - C2014

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