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C1999

Sigma-Aldrich

CMP-Sialic Acid Synthetase from Neisseria meningitidis group B

recombinant, expressed in E. coli BL21, ≥10 units/mg protein

Sinonimo/i:

CTP: N-Acylneuraminate cytidylyltransferase

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About This Item

Classificazione EC (Enzyme Commission):
2.7.7.43 (BRENDA, IUBMB)
Numero MDL:
MFCD01323074
Codice UNSPSC:
12352204
NACRES:
NA.54

Ricombinante

expressed in E. coli BL21

Livello qualitativo

200

Forma fisica

lyophilized solid

Attività specifica

≥10 units/mg protein

PM

26.0 kDa

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Cytidine monophosphate (CMP)-Sialic Acid Synthetase from Neisseria meningitidis group B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.

Applicazioni

The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.

Azioni biochim/fisiol

Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion of N?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+ is essential for the catalytic functionality of CMP-sialic acid synthetase.

Definizione di unità

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 °C at pH 8.0.

Stato fisico

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

Risultati analitici

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 °C for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Jessica H Wong et al.
Organic & biomolecular chemistry, 7(1), 27-29 (2008-12-17)
A modular replacement approach to the synthesis of sulfo-nucleotide analogs prepared from condensation of nucleoside aldehydes with bis phosphonate Horner-Wadsworth-Emmons reagents is disclosed. These analogs were shown to be inhibitors of Neisseria meningitidis CSS (NmCSS), which is a key enzyme
Hai Yu et al.
Bioorganic & medicinal chemistry, 12(24), 6427-6435 (2004-11-24)
Three C terminal His6-tagged recombinant microbial CMP-sialic acid synthetases [EC 2.7.7.43] cloned from Neisseria meningitidis group B, Streptococcus agalactiae serotype V, and Escherichia coli K1, respectively, were evaluated for their ability in the synthesis of CMP-sialic acid derivatives in a
Purification and characterization of the recombinant CMP-sialic acid synthetase from Neisseria meningitides.
Gilbert, M., et al
Biotechnology Letters, 19, 417-420 (1997)
CMP-sialic acid synthetase: the point of constriction in the sialylation pathway
SialoGlyco Chemistry and Biology I, 139-167 (2013)
Sandra Ratzow et al.
Journal of clinical microbiology, 45(6), 1965-1968 (2007-04-06)
The standard sequence-based method for the typing of Legionella pneumophila serogroup 1 strains was extended by using the gspA and neuA alleles. The use of neuA as a seventh allele for typing significantly increased the index of discrimination calculated for
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