S1951
α-2,3-Sialyltransferase from Pasteurella multocida
recombinant, expressed in E. coli BL21, ≥2 units/mg protein
Sinonimo/i:
CMP-N-acetylneuraminate:β-D-galactoside α-(2,3)-N-acetylneuraminyltransferase
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 unit | Per informazioni sulla disponibilità, contatta il Servizio Clienti. | 289,00 € |
Informazioni su questo articolo
Specific activity:
≥2 units/mg protein
Recombinant:
expressed in E. coli BL21
289,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Servizio Tecnico
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Permettici di aiutartirecombinant
expressed in E. coli BL21
Quality Segment
form
lyophilized powder
specific activity
≥2 units/mg protein
mol wt
46.4 kDa
shipped in
dry ice
storage temp.
−20°C
General description
Sialyltransferases belongs to the glycosyltransferases family with the capability of catalyzing the transfer of N-acetylneuraminic acid residues.[1] It is a multifunctional enzyme with α-2,3-sialyltransferase activity, α-2,6-sialyltransferase activity, sialidase activity, and trans-sialidase activity.[2] It has a molar mass of 46.4 kDa, with pI, pH being 5.94 and 7.5-8.5 respectively.
Biochem/physiol Actions
Sialyltransferase catalyzes the transfer of Neu5Ac from CMP-Neu5Ac as a donor substrate to the β-D-galactosyl-1,4-N-acetyl-D-glucosaminyl termini of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.[1]
Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.
Physical form
Lyophilized powder containing Tris-HCl and NaCl
Preparation Note
Reconstitute the lyophilized powder with a volume of water in the range of 0.1 mL to 1 mL, to give a concentration in the range of 1 unit/mL (1 mL volume of water) to 10 units/mL (0.1 mL volume of water).
Analysis Note
Enzymatic activity assays performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5Ac (1 mM) and Lac-β−OMU (1 mM) at 37°C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm).
Other Notes
One unit will catalyze the formation of 1.0 μmol Neu-5-Ac-α-2,3LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.
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Questo articolo | |||
|---|---|---|---|
| specific activity ≥2 units/mg protein | specific activity ≥5 units/mg protein | specific activity ≥3.0 units/mg protein | specific activity ≥10 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized solid |
| recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 | recombinant expressed in E. coli BL21 |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| mol wt 46.4 kDa | mol wt 56.8 kDa | mol wt 33.4 kDa | mol wt 26.0 kDa |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Classe di stoccaggio
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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