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77618

Sigma-Aldrich

Miscela di fenolo-cloroformio-alcol isoamilico

BioUltra, for molecular biology, 49.5:49.5:1

Sinonimo/i:

phenol:Chloroform:Isoamyl Alcohol

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About This Item

Numero CAS:
136112-00-0
Numero MDL:
MFCD00133763
Codice UNSPSC:
12352401
ID PubChem:
329767817
NACRES:
NA.07
Grado:
for molecular biology

Grado

for molecular biology

Livello qualitativo

100

Nome Commerciale

BioUltra

Stato

liquid

Impurezze

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

7.7-8.3(H2O-phase, after extraction with H2O, 1:1)

Densità

1.29 g/mL at 20 °C

Assorbimento

cut-off at 360 nm

Temperatura di conservazione

2-8°C

Stringa SMILE

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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Applicazioni


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems.: This study highlights the use of a phenol-chloroform-isoamyl alcohol mixture for the extraction and quantification of RNA cargos from nanoparticles, facilitating accurate delivery system analysis (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry.: This research utilized the phenol-chloroform-isoamyl alcohol mixture to extract microRNAs from serum samples, providing insights into cancer biomarkers (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs.: The study demonstrates the effectiveness of using phenol-chloroform-isoamyl alcohol for DNA extraction in forensic applications, improving the analysis of mixed samples (Nori and McCord, 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues.: This article emphasizes the necessity of phenol-chloroform-isoamyl alcohol extraction in accurately detecting mycobacterial DNA in tissue samples, enhancing diagnostic precision (Radomski et al., 2013).

Stato fisico

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 49.5:49.5:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

Altre note

Large- and small-scale phenol extractions

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Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Organi bersaglio

Central nervous system, Liver,Kidney, Nervous system,Kidney,Liver,Skin

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

177.8 °F - closed cup

Punto d’infiammabilità (°C)

81 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCL8747
BCCJ5271
BCCB6849
BCCG5579
BCBW2879

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Large- and small-scale phenol extractions.
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