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646563

Supelco

DL-Dithiothreitol solution

1 M in H2O

Sinonimo/i:

(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT

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10 X 0.5 ML
109,00 €

109,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
10 X 0.5 ML
109,00 €

About This Item

Formula empirica (notazione di Hill):
C4H10O2S2
Numero CAS:
3483-12-3
Peso molecolare:
154.25
Beilstein:
1719757
Numero MDL:
MFCD00004877
Codice UNSPSC:
12161504
ID PubChem:
24883472
NACRES:
NA.25

109,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Richiedi un ordine bulk

Stato

liquid

Livello qualitativo

100

Impiego in reazioni chimiche

reagent type: reductant

Concentrazione

1 M in H2O

Densità

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

Stringa SMILE

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
VHJLVAABSRFDPM-QWWZWVQMSA-N

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Descrizione generale

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Applicazioni

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Caratteristiche e vantaggi

Low toxicity and reduced odor compared to other thiol compounds.

Accessorio

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H315,H318

Classi di pericolo

Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
MKCW8841
MKCV9188
MKCV8762
MKCW6078
MKCV6891

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L-(−)-Dithiothreitol ≥95% (titration)

Sigma-Aldrich

D9760

L-(−)-Dithiothreitol

Iodoacetamide Single use vial of 56 mg

Sigma-Aldrich

A3221

Iodoacetamide

Sputolysin® Reagent Intended for the isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum.

Millipore

560000

Sputolysin® Reagent

DTT OmniPur® Grade, >= 99.4%, Used for reduction of protein disulfide linkages

Millipore

3860-OP

DTT

Willem-Jan M Schellekens et al.
Critical care (London, England), 18(1), R28-R28 (2014-02-11)
Diaphragm weakness induced by prolonged mechanical ventilation may contribute to difficult weaning from the ventilator. Hypercapnia is an accepted side effect of low tidal volume mechanical ventilation, but the effects of hypercapnia on respiratory muscle function are largely unknown. The
Calista K L Ng et al.
Human molecular genetics, 24(11), 3163-3171 (2015-02-26)
mRNA decay is an essential and active process that allows cells to continuously adapt gene expression to internal and environmental cues. There are two mRNA degradation pathways: 3' to 5' and 5' to 3'. The DCPS protein is the scavenger
Yukari Yamane-Sando et al.
MicrobiologyOpen, 3(2), 196-212 (2014-02-11)
Sphingolipids are a family of eukaryotic lipids biosynthesized from sphingoid long-chain bases (LCBs). Sphingolipids are an essential class of lipids, as their depletion results in cell death. However, acute LCB supplementation is also toxic; thus, proper cellular LCB levels should
I Dzneladze et al.
Leukemia, 29(7), 1485-1495 (2015-03-05)
In this study, we investigated the role of inositol polyphosphate-4-phosphatase, type-II (INPP4B) in acute myeloid leukemia (AML). We observed that AML patients with high levels of INPP4B (INPP4B(high)) had poor response to induction therapy, shorter event-free survival and shorter overall
Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
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Domande

1–6 di 6 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  3. after opening the ampule is DTT still usable or only single use?

    1 risposta

    1. This product is intended for single use. The stability of the solution after opening the ampule has not been determined. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/629/609/646563dat.pdf

      Utile?

  4. how to use DDT

    1 risposta

    1. 646563 (DL-Dithiothreitol solution, DTT) could be applied for research, such as western blot analysis, lysis buffer, etc. The procedure of SDS-PAGE sample preparation with DTT has been listed in the datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/629/609/646563dat.pdf
      For other applications, please check the "Peer Reviewed Papers" section on the web for more literature: https://www.sigmaaldrich.com/product/sigma/646563

      Utile?

  5. What is the expiration time for this product?

    1 risposta

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Utile?

  6. What are the storage conditions for this product?

    1 risposta

    1. This product is stored in room temperature. Store tightly closed in a dry and well-ventilated place. Please see section 7 of the Safety Data Sheet for more information: https://www.sigmaaldrich.com/sds/sigma/646563

      Utile?

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