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21888

Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Sinonimo/i:

CFSE

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25 MG
430,00 €
100 MG
472,00 €

430,00 €

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25 MG
430,00 €
100 MG
472,00 €

About This Item

Formula empirica (notazione di Hill):
C29H19NO11
Numero CAS:
150347-59-4
Peso molecolare:
557.46
Numero MDL:
MFCD00037466
Codice UNSPSC:
12352108
ID PubChem:
57647951
NACRES:
NA.32

430,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Richiedi un ordine bulk

Nome Commerciale

BioReagent

Livello qualitativo

200

Saggio

≥90% (HPLC)

Stato

solid

Punto di fusione

152-154 °C (lit.)

Solubilità

DMF: soluble
DMSO: soluble

Fluorescenza

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Stringa SMILE

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3
LZFDYQHOQDFMOA-UHFFFAOYSA-N

Descrizione generale

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.[1]

Applicazioni

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry.[2][3] Forms green fluorescent conjugates on deacetylation.
5[4]-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry[5][6]
  • in lymphocyte proliferation assay[7][8]
  • to determine the intracellular or intra-organellar pH in chloroplasts[4]
  • to label Leishmania amazonensis and Leishmania major[9]

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Azioni biochim/fisiol

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.[10]

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

06693

Timestrip Plus™ -20 °C

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
BCCF7480
BCCK2345
BCCC4532
BCBW9190
BCBT5235

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Microbial Food Safety Along the Dairy Chain (2017)
WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
Autophagic induction greatly enhances Leishmania major intracellular survival compared to Leishmania amazonensis in CBA/j-infected macrophages
Dias BRS, et al.
Frontiers in Microbiology, 9 (2018)
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Articoli

Saggi di proliferazione e vitalità cellulare

Saggi basati su cellule per valutare la proliferazione cellulare (BrdU, MTT, WST1), la vitalità cellulare e la citotossicità nel campo della ricerca oncologica, neuroscientifica e delle cellule staminali.

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Domande

1–6 di 6 domande  

  1. What is the in vivo (mouse) half-life of CFDA-SE? What is the route of clearance (kidney, liver?)

    1 risposta

    1. The half-life is not determined for this product.
      Please see the link below to a publication that may be helpful:
      https://www.semanticscholar.org/paper/The-Use-of-Carboxyfluorescein-Diacetate-Ester-to-Quah-Parish/cae3435e063cb9f46cea4ddfc7c560f5821e19b3

      Please note that, unless otherwise stated, this product is intended for research use only and is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

      Utile?

  2. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  3. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?

    1 risposta

    1. No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.

      Utile?

  4. At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?

    1 risposta

    1. We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.

      Utile?

  5. What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?

    1 risposta

    1. Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.

      Utile?

  6. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?

    1 risposta

    1. Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.

      Utile?

Recensioni

Nessuna valutazione

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