14562
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester
BioReagent, for fluorescence
Sinonimo/i:
BCECF-AM
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1 MG
255,00 €
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1 MG
255,00 €
About This Item
Formula empirica (notazione di Hill):
C39H36O19
Peso molecolare:
808.69
Numero MDL:
Codice UNSPSC:
12352108
NACRES:
NA.32
Prodotti consigliati
Grado
for fluorescence
Nome Commerciale
BioReagent
Stato
solid
Solubilità
DMF: soluble
DMSO: soluble
acetonitrile: soluble
Fluorescenza
λex 482 nm; λem 528 nm in 0.1 M Tris pH 8.0 (esterase)
Compatibilità
suitable for fluorescence
Temperatura di conservazione
2-8°C
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Applicazioni
An uncharged, nonfluorescent molecule that is useful for noninvasive bulk loading of cell suspension because it is capable of permeating the cell membrane. Cellular nonspecific esterases cleave its lipophilic blocking groups, resulting in a charged, fluorescent form of the molecule. Compared to the parent compound, this charged derivative leaks out of the cell at a much slower rate. Utilized for pH measurements in perfused tissues[1][2][3], intercellular spaces[4][5], mammalian cells[6][7][8], plant cells[9], bacteria[10][11], and yeast[12][13]. Also, employed to investigate in assays for cellular functional properties such as adhesion[14][15][16][17][18][19], multi-drug resistance[20][21][22], viability and cytotoxicity[23][24][25][26][27], apoptosis[28] and chemotaxis[15].
Cell-permeable ester of BCECF that, on hydrolysis by cytosolic esterases, yields the intracellularly trapped pH-indicator BCECF. Allows simultaneous recording of cell volume changes and intracellular pH in single osteosarcoma cells.
Confezionamento
Bottomless glass bottle. Contents are inside inserted fused cone.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
H W van Veen et al.
The Journal of biological chemistry, 269(47), 29509-29514 (1994-11-25)
The strictly aerobic, polyphosphate-accumulating Acinetobacter johnsonii strain 210A degrades its polyphosphate when oxidative phosphorylation is impaired. The endproducts of this degradation, divalent metal ions and inorganic phosphate, are excreted as a neutral metal-phosphate (MeHPO4) chelate via the electrogenic MeHPO4/H+ symport
M T Alonso et al.
The Biochemical journal, 272(2), 435-443 (1990-12-01)
The effects of arachidonic acid and thrombin on calcium movements have been studied in fura-2-loaded platelets by a procedure which allows simultaneous monitoring of the uptake of manganese, a calcium surrogate for Ca2+ channels, and the release of Ca2+ from
R S Haworth et al.
Biochimica et biophysica acta, 1098(1), 79-89 (1991-12-13)
We have tested the efficacy of fluorescent probes for the measurement of intracellular pH in Saccharomyces cerevisiae. Of the compounds tested (fluorescein, carboxyseminaphthorhodafluor-1 (C.SNARF-1) and 2',7'bis(carboxyethyl)-5(6')-carboxyfluorescein), C.SNARF-1 was found to be the most useful indicator of internal pH. Fluorescence microscopy
P J Harris et al.
The American journal of physiology, 266(1 Pt 1), C73-C80 (1994-01-01)
The lateral intercellular spaces (LIS) of reabsorptive epithelia are the site of the proposed local osmotic gradient responsible for transepithelial transport. We developed techniques for loading the LIS of living cultured renal cells (MDCK and LLC-PK1) with the fluorescent dye
J T Seo et al.
Pflugers Archiv : European journal of physiology, 426(1-2), 75-82 (1994-01-01)
Intracellular pH (pHi) has been measured in intact, perfused rat mandibular salivary glands loaded with the fluorescent pH indicator BCECF [2',7'-bis(2-carboxyethyl)-5(6)- carboxyfluorescein]. Glands mounted in the cuvette of a conventional bench-top spectro-fluorometer were perfused for 5 min with the acetoxymethyl
Articoli
Fluorescence lifetime measurement is advantageous over intensity-based measurements. Applications include fluorescence lifetime assays, sensing and FLI.
Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.
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