C1359

Calcein AM solution

≥90% purity (HPLC), solution

• Formula empirica (notazione di Hill): C₄₆H₄₆N₂O₂₃
• Numero CAS: 148504-34-1
• Peso molecolare: 994.86
• Numero CE: 200-664-3
• Numero MDL: MFCD05861516
• Codice UNSPSC: 12171500
• ID PubChem: 24892405
• NACRES: NA.47

Proprietà

• Nome del prodotto: Calcein AM solution, 4 mM in DMSO, ≥90% (HPLC), solution
• Livello qualitativo: 100
• Saggio: ≥90% (HPLC)
• Stato: solution
• Concentrazione: 4 mM in DMSO
• tecniche: titration: suitable
• Colore: colorless; white to off-white
• λmax: 491-497 nm
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: −20°C
• Stringa SMILE: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3; XKFSBWQWNMZWFA-UHFFFAOYSA-N

Descrizione

Applicazioni

In cells, calcein AM solution has been used in calcein-AM dye transfer assay.[1] It has been used for the staining of cells to study quantification of cell-migration.[2] It has been used to study cytotoxic effects of drugs in cells.[3]

Azioni biochim/fisiol

Calcein AM is a membrane permeable nonfluorescent molecule. It is converted to fluorescent calcein by intracellular esterases. Calcein has limited membrane permeability. Calcein AM is also a substrate for p-glycoprotein (Pgp). Presence of Pgp results in efflux of calcein AM and thereby decreases cellular fluorescence. On the other hand, presence of Pgp inhibitor increases cellular entry of calcein AM and thereby increases cellular fluorescence. Calcein AM plays an important role in early drug discovery.[4]

Fluorescent cell permeable derivative of calcein.

Stato fisico

Solution in 0.4% Calcein AM and 99.6% DMSO.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): 185.0 °F - closed cup
• Punto d’infiammabilità (°C): 85 °C - closed cup
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)