11412272001

Transforming Growth Factor-β1, human (hTGF-β1)

recombinant (CHO cells)

• Sinonimo/i: hTGF
• Codice UNSPSC: 12352207

Proprietà

• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in CHO cells
• Saggio: >95% (SDS-PAGE)
• Stato: solution
• Potenza: <0.1 ng/mL EC₅₀
• PM: 25,000 Da
• Confezionamento: pkg of 1 mL (1 μg)
• Produttore/marchio commerciale: Roche
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• Impurezze: <0.1 EU/μgtested (LAL test)
• N° accesso UniProt: P01137
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... TGFB1(7040)

Descrizione

Descrizione generale

Recombinant, human TGF- β1 is produced in CHO (Chinese Hamster Ovary) cells and purified by standard chromatographic techniques.
Solution in PBS and 1 mg/ml BSA, filtered through 0.2 μm pore size membrane.

Specificità

Species specificity: Transforming growth factor-β1 (TGF-β1) is effective on human and mouse cells.
Specific activity/EC₅₀: <0.1 ng/ml, at least the same specific activity (EC<SUB>50) compared to the indicated standard is guaranteed.

Applicazioni

Transforming growth factor-β1, human (hTGF-β1) has been used to explore function and underlying mechanism of periostin (PN) during orthodontic periodontal ligament remodeling.[1]

Azioni biochim/fisiol

Transforming Growth Factor-β1, human (hTGF-β1), is a multifunctional growth factor affecting many functions in a variety of different cells.[2]

Transforming growth factor-β (TGF-β) family comprises of a group of multifunctional cytokines which are involved in controlling apoptosis, growth and differentiation of cells, and play important role during embryo development.[3]

Qualità

Purity: >95% (SDS-PAGE)
Endotoxin level: <0.1 EU/μg (LAL-test), <10 EU/ml (LAL-test)
(Note: 1 EU corresponds to 0.1 ng)

Sequenza

Chain Length 2 x 112 AA

Two identical polypeptide chains (112 amino acids) connected by disulfide bridges, identical to natural hTGF-β1.

Definizione di unità

EC₅₀ definition: The concentration of hTGF-β1 that is required to mediate half maximal inhibition of DNA synthesis (BrdU incorporation) with Mv1-Lu cells.

Nota sulla preparazione

Working concentration: 0.01 - 10 ng/ml
Recombinant, human TGF-β 1 exerts its biological activity in the concentration range of 0.01–10 ng/ml.
Working solution: Dilute the concentrated TGF-β1 solution (1 μg/ml) with PBS or culture medium containing BSA [or HSA (human serum albumin)], 1 mg/ml (0.1%) or serum 1–10%.
Storage conditions (working solution): For best results, prepare appropriate aliquots and store at -15 to -25 °C.
Note: Avoid repeated freezing and thawing.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash