10280879001
Roche
5-bromo-2′-deossiuridina
>97%, crystalline solid, pkg of 1 g
Sinonimo/i:
5-BrdU, 5-bromo-1-(2-deossi-β-D-ribofuranosil)uracile, 5-bromouracil deossiriboside, BUdR
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About This Item
Formula empirica (notazione di Hill):
C9H11BrN2O5
Numero CAS:
Peso molecolare:
307.10
Beilstein:
30395
Numero MDL:
Codice UNSPSC:
12352204
ID PubChem:
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Livello qualitativo
Saggio
>97%
Stato
crystalline solid
PM
307.1
Confezionamento
pkg of 1 g
Produttore/marchio commerciale
Roche
Punto di fusione
191-194 °C (dec.) (lit.)
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Stringa SMILE
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
WOVKYSAHUYNSMH-RRKCRQDMSA-N
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Applicazioni
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle[3] and primary hepatocytes during cell proliferation studies.[4]
Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis[5] and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
Azioni biochim/fisiol
Analogo della timidina usato come mutageno nella ricerca genetica. Incorporato selettivamente nel DNA cellulare durante la fase S.
Qualità
Purity: 97% (from N), chromatographically homogeneous
Nota sulla preparazione
Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
Altre note
For life science research only. Not for use in diagnostic procedures.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Muta. 1B - Repr. 2
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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