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SCM075

Sigma-Aldrich

Inibitore ROCK (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Sinonimo/i:

Y-27632

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About This Item

Codice UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Livello qualitativo

100

Forma fisica

liquid

PM

320.26 g/mol

tecniche

cell culture | mammalian: suitable

Condizioni di spedizione

dry ice

Descrizione generale

L′inibitore ROCK (Y-27632) è un inibitore selettivo, ATP-competitivo della proteina chinasi associata a Rho (ROCK) che include p160Rock (Ki=140 nM) e Rock-II. Agisce anche in qualità di potente inibitore della sensibilizzazione della fosforilazione della miosina e della contrazione del muscolo liscio da parte del Ca2+ indotta dall′agonista. Un′ora di trattamento con Y-27632 10 µM blocca l′apoptosi delle cellule staminali embrionali umane (hESC) in coltura dissociate, aumenta la sopravvivenza e l′efficienza della clonazione di hESC senza comprometterne la pluripotenzialità1. Recentemente, alcuni report hanno mostrato che l′applicazione di Y-27632 e Accutasi (n. cat. SCR005), una miscela di enzimi digestivi in formula, migliora il tasso di sopravvivenza delle singole hESC dopo lo scongelamento usando i metodi convenzionali di congelamento e scongelamento lento.

Stoccaggio e stabilità

Prima della ricostituzione: Mantenere a -20 ºC, al riparo dalla luce per 2 anni. Dopo la ricostituzione: 1 anno a -20 ºC, al riparo dalla luce

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Malgorzata Bienkowska-Haba et al.
Journal of virology, 94(3) (2019-11-22)
It is established that the host cell transcriptomes of natural lesions, organotypic rafts, and human papillomavirus (HPV)-immortalized keratinocytes are altered in the presence of HPV genomes. However, the establishment of HPV-harboring cell lines requires selection and immortalization, which makes it
Maike Frye et al.
eLife, 9 (2020-09-09)
Endothelial integrity is vital for homeostasis and adjusted to tissue demands. Although fluid uptake by lymphatic capillaries is a critical attribute of the lymphatic vasculature, the barrier function of collecting lymphatic vessels is also important by ensuring efficient fluid drainage
Ambra Gianotti et al.
Frontiers in pharmacology, 9, 1176-1176 (2018-11-13)
Cystic fibrosis (CF) is a chronic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, which encodes for a channel expressed at the apical surface of epithelial tissues. Defective chloride and bicarbonate secretion, arising from CFTR mutations
Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
Fibrosis is characterized by hardening, overgrowth, and development of scars in various tissues as a result of faulty reparative processes, diseases, or chronic inflammation. During the fibrotic process in the corneal stroma of the eye, the resident cells called keratocytes
Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
Fibroblasts transformed by the proto-oncogene Src form individual invadopodia that can spontaneously self-organize into large matrix-degrading superstructures called rosettes. However, the mechanisms by which the invadopodia can spatiotemporally reorganize their architecture is not well understood. Here, we show that Hic-5
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