8.51025

5-Carboxyfluorescein

Novabiochem^®

• Sinonimo/i: 5-Carboxyfluorescein, 5-FAM
• Formula empirica (notazione di Hill): C₂₁H₁₂O₇
• Numero CAS: 76823-03-5
• Peso molecolare: 376.32
• Numero MDL: MFCD00036874
• Codice UNSPSC: 12352005
• NACRES: NA.22

Proprietà

• Livello qualitativo: 200
• Nome Commerciale: Novabiochem^®
• Saggio: ≥98% (TLC); ≥99.0% (HPLC)
• Stato: powder
• Impiego in reazioni chimiche: reagent type: fluorescent-labelling reagent
• Produttore/marchio commerciale: Novabiochem^®
• applicazioni: peptide synthesis
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: O1C3(c4c(cc(cc4)O)Oc5c3ccc(c5)O)c2c(cc(cc2)C(=O)O)C1=O
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25); NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descrizione

Descrizione generale

This high purity fluorescent dye (λ_ex494 nm, λ_em 518 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization. FAM should be introduced as the last step in the synthesis to avoid formation of side-products, or be protected by tritylation prior to acylation or hydrazine treatment ^[1].

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Literature references

^[1] R. Fischer, et al. (2003) Bioconjugate Chem., 14, 653.

Applicazioni

5-Carboxyfluorescein Novabiochem^®. CAS 76823-03-5, molar mass 376.32 g/mol.

Linkage

Replaces: 01-63-0112

Risultati analitici

Colour (visual): yellow to orange
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 98 %
Assay (HPLC, area%): ≥ 99.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem^® products please click here.

Note legali

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable