06-1082-I

Anti-IL-27A Antibody

from rabbit, purified by affinity chromatography

• Sinonimo/i: Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, Interleukin-30, p28, IL-27A
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Purificato mediante: affinity chromatography
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• N° accesso NCBI: NP_663634
• N° accesso UniProt: Q8NEV9
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... IL27(246778)

Descrizione

Descrizione generale

Interleukin 27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits: Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-27A). IL-27 has pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. IL-27 is secreted by antigen-presenting cells and plays an important function in regulating the activity of B and T lymphocytes. IL-27 suppresses the expression of GATA3, the inhibitor of TH1 cell development. In addition to suppressing cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its anti-tumor activity as well as its anti-angiogenic activity with activation of production of anti-angiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increases the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication.

Immunogeno

KLH-conjugated linear peptide corresponding to human IL-27A.

Applicazioni

Anti-IL-27A Antibody is an antibody against IL-27A for use in Western Blotting, Immunohistochemistry and Flow Cytometry.

Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected IL-27A in human placenta and human tonsil tissue.
Flow Cytometry Analysis: 0.5 µg of this antibody from a representative lot detected IL-27A in 1X10E6 staph enterotoxin B treated human peripheral blood mononuclear cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Qualità

Evaluated by Western Blotting in human placenta tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-27A in 10 µg of human placenta tissue lysate.

Descrizione del bersaglio

~27 kDa observed. Uncharacterized band(s) may appear in some lysates.

Linkage

Replaces: 06-1082

Stato fisico

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable