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F2456

Sigma-Aldrich

Fluorescein (free acid)

Dye content 95%, Powder

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About This Item

Formula empirica (notazione di Hill):
C20H12O5
Numero CAS:
2321-07-5
Peso molecolare:
332.31
Colour Index:
45350:1
Beilstein:
94324
Numero CE:
219-031-8
Numero MDL:
MFCD00005050
Codice UNSPSC:
12171500
ID PubChem:
24894822
NACRES:
NA.47

product name

Fluorescein (free acid), Dye content 95 %

Forma fisica

powder

Livello qualitativo

200

Composizione

Dye content, 95%

Colore

orange to red-brown

Punto di fusione

320 °C (lit.)

λmax

496 nm

ε (coefficiente d’estinzione)

≥12000 at 282-286 nm
≥39000 at 236-240 nm
≥70000 at 488-492 nm

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
GNBHRKFJIUUOQI-UHFFFAOYSA-N

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Descrizione generale

Fluorescein (free acid, CI 45350) is a xanthene dye. Fluorescein (free acid) molecule can be divided into two parts. One part contains xanthene moiety which acts as fluorophore and the other part of the molecule bears benzene moiety which is the fluorescence-controlling moiety.

Applicazioni

Fluorescein (free acid) has been used as a staining agent in retinal imaging.
Fluorescein (free acid) is widely used as a fluorescent stain and trace for many living cells and tissue applications. It is also suitable as a marker for the study of motility of bile canaliculi. Some of the clinical applications are evaluating the ocular surface and delineation of brain regions lacking a blood-brain barrier to permit accurate surgical resection of malignant glioma. Fluorescein angiography helps investigate changes in retinal blood supply. Fluorescein also plays a role as a physiological tracer to characterize the salmon′s intestinal permeability (Schep et al., 1998), and for the fluorophotometric measurement of aqueous humor flow. It is widely employed as a quantum yield standard.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H319

Consigli di prudenza

P305 + P351 + P338

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Tasuku Ueno et al.
Journal of the American Chemical Society, 126(43), 14079-14085 (2004-10-28)
Rational design strategies based on practical fluorescence modulation mechanisms would enable us to rapidly develop novel fluorescence probes for target molecules. Here, we present a practical and general principle for modulating the fluorescence properties of fluorescein. We hypothesized that (a)
Cunyang Wang et al.
Materials science & engineering. C, Materials for biological applications, 103, 109823-109823 (2019-07-28)
Acrylic bone cement (ABC) has been used as a grouting agent in joint replacement surgery for over 50 years. In particular, ABC is irreplaceable for high-load joint replacement such as total hip joint replacements (THJRs) and total knee joint replacements because
Han Liu et al.
Journal of ethnopharmacology, 241, 111975-111975 (2019-05-30)
Danhong Huayu Koufuye (DHK), a compound traditional Chinese medicine, is composed of Salvia miltiorrhiza radix (Salvia miltiorrhiza Bge.), Angelicae Sinensis radix (Angelicae Sinensis (Oliv.) Diels.), Chuanxiong rhizoma (Ligusticum chuanxiong Hort.), Persicae semen (Prunus persica (L.) Batsch), Carthami flos (Carthamus tinctorius
Karen A Fisher et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 9(23), 5873-5878 (2003-12-16)
The uptake of the three species of the drug model fluorescein (fluorescein sodium salt (FNa), fluorescein free acid (F), and fluorescein diacetate (FDA)) by zeolite NaX and the mesoporous zeotype MCM-41 was investigated as well as their release rates into
Mi Ju Son et al.
Medicine, 98(29), e16527-e16527 (2019-07-25)
Several studies have found that obesity is associated with atopic dermatitis (AD); however, the mechanisms underlying the association are largely unknown. This study aims to assess the association of AD with obesity in the Korean population and verify its mechanism
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