While the specifications for this probe do not include assaying for specific applications, there are many publications showing its use in flow cytometry applications:
https://www.sciencedirect.com/science/article/pii/S0022030215000909
https://iubmb.onlinelibrary.wiley.com/doi/pdf/10.1002/iub.1895
https://www.tandfonline.com/doi/pdf/10.1080/21655979.2021.1994737
https://journal.waocp.org/article_91422_6ccd2a19eb9159073edeb8f9ce0be660.pdf
https://pmc.ncbi.nlm.nih.gov/articles/PMC7842666/
D6883
2′,7′-diclorofluorescina diacetato
≥97% (HPLC), powder, cell-permeable non-fluorescent probe
Sinonimo/i:
DCFH-DA
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Cambia visualizzazione
50 MG
61,50 €
250 MG
246,00 €
About This Item
Formula empirica (notazione di Hill):
C24H16Cl2O7
Numero CAS:
Peso molecolare:
487.29
Beilstein:
4610273
Numero MDL:
Codice UNSPSC:
12352200
ID PubChem:
NACRES:
NA.77
Prodotti consigliati
Nome del prodotto
2′,7′-diclorofluorescina diacetato, ≥97%
Livello qualitativo
Saggio
≥97%
Stato
powder
Temperatura di conservazione
−20°C
Stringa SMILE
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
PXEZTIWVRVSYOK-UHFFFAOYSA-N
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Descrizione generale
La 2′,7′-diclorodiidrofluoresceina diacetato (DCFH-DA) è un colorante fluorescente sensibile all'ossidazione, utilizzato per rilevare il livello intracellulare di specie reattive dell'ossigeno (ROS). Il DCFH-DA è una sonda non fluorescente in grado di permeare le cellule, che può essere idrolizzata dall'esterasi intracellulare a dare DCFH, che per ossidazione da parte dei ROS intracellulari forma DCF fluorescente.[1]
The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.
Applicazioni
La 2′,7′-diclorofluoresceina diacetato trova impiego:
- nella quantificazione rapida e sensibile delle specie reattive dell′ossigeno in risposta al metabolismo ossidativo;[2][1][3]
- nei saggi su micropiastra per rilevare i prodotti ossidativi nelle cellule fagocitiche;
- nei saggi quantitativi di differenziazione mieloide in piastre multipozzetto
Trova impiego nella quantificazione rapida e sensibile delle specie reattive dell′ossigeno in risposta al metabolismo ossidativo, nei saggi su micropiastre per il rilevamento dei prodotti ossidativi nelle cellule fagocitiche e nei saggi quantitativi di differenziazione mieloide su piastre multipozzetto.
Stoccaggio e stabilità
Questo prodotto deve essere conservato in polvere a -20 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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Articoli
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DISCOVER Bioactive Small Molecules for Nitric Oxide & Cell Stress Research
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Is D6883 2´,7´-Dichlorofluorescin diacetate suitable for flow cytometry analyses?
1 risposta-
Utile?
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What is the protocol for measuring ROS using D6883? Specifically, what kind of media or buffer is used with this product?
1 risposta-
The specific protocol for measuring ROS using D6883 and the type of media or buffer to be used with this product are not available. However, typically, this product is added to a buffer solution shortly before assessing the fluorescent signal. For further guidance, refer to the following citations for relevant information:
1. Novel Antioxidant Tripeptide "ACQ" Can Prevent UV-Induced Cell Death and Preserve the Number of Epidermal Stem Cells
PMID: 261996772. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2',7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937
PMID: 2057064These publications may provide insights into the protocol for using D6883 and the type of media or buffer used with this product.
Utile?
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What concentration of DCF can I use for A549 cells?
1 risposta-
This product has not been tested for use in cell culture. A search of the literature suggests working concentrations range from 5 µM to 0.1 mM. See the link below to a publication that specifically looked at A549 cells using several dyes, including DCFDA:
https://pubs.rsc.org/en/content/articlehtml/2017/ra/c7ra01025gUtile?
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What concentration of DCF can I use for THP-1 monocyte cell line?
1 risposta-
The optimal concentration of 2′,7′-Dichlorofluorescin diacetate (DCFDA) for use with THP-1 monocytes will need to be determined by the end-user. See the link below to a paper that reported using DCF at 40 uM with THP-1 cells.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8961584/Utile?
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Is it possible to view this probe under a fluorescence microscope if the probe is solubilised in DMSO? What are the excitation and absorption wavelengths?
1 risposta-
The excitation wavelength is 504 nm and the emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however, various publications suggest that it is suitable. See the link below for an example:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/Utile?
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Protocol please! Thank you very much!
1 risposta-
It may be helpful to view the following articles as there is not a specific protocol for this product:
Utile?
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Can the product be stored in powder form and if its possible what is the storage temp?
1 risposta-
This product should be stored in powdered format at -20°C.
Utile?
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What is the solution stability of Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 risposta-
This product can be stored at -20°C for up to six months in solvents such as DMSO, ethanol, or DMF (N,N-dimethylformamide).
Utile?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Utile?
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What is the excitation and emission wavelength for Product D6883, 2´,7´-Dichlorofluorescin diacetate?
1 risposta-
The excitation wavelength is 504 nm and emission wavelength is 529 nm (in ethanol).
Utile?
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