SHC007
MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA
shRNA sequence targeting luciferase
Szinonimák:
MISSION® Control Vectors
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
Minőségi szint
termékcsalád
MISSION®
koncentráció
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
kiszállítva
dry ice
tárolási hőmérséklet
−20°C
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Általános leírás
The MISSION® Luciferase shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting luciferase from Photinus pyralis (GenBank Accession No. M15077). The Luciferase shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing firefly luciferase. It can also be used as a negative control vector.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The Luciferase shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids, MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The Luciferase shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Alkalmazás
To see more application data, protocols, vector maps visit sigma.com/shrna.
Jogi információk
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
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