S0698

Anti-SmB antibody, Mouse Monoclonal

clone 12F5, purified from hybridoma cell culture

• Szinonimák: Anti-SNRPB1, Anti-Sm protein B, Anti-Sm-B, Anti-SmB/SmB, Anti-Small nuclear ribonucleoprotein associated protein B, Anti-snRNP-B
• UNSPSC kód: 12352203
• NACRES: NA.41
• konjugátum: unconjugated
• application: ELISA (i); ICC; IP; WB
• klón: 12F5, monoclonal
• faj reaktivitás: human, mouse, canine
• citations: 15
• technika/technikák: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear cell extract

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: purified from hybridoma cell culture
• antitest terméktípus: primary antibodies
• klón: 12F5, monoclonal
• molekulatömeg: antigen ~25 kDa
• faj reaktivitás: human, mouse, canine
• technika/technikák: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.5-1 μg/mL using HeLa nuclear cell extract
• izotípus: IgG1
• UniProt elérési szám: P14678
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... SNRPB(6628) ; mouse ... Snrpb(20638)

Leírás

Általános leírás

Monoclonal Anti-SmB (mouse IgG1 isotype) is derived from the hybridoma 12F5-H3.D8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with an SmB recombinant protein.

SNRPB (small nuclear ribonucleoprotein-associated protein B) is an important constituent of the spliceosome. It codes for SmB/B′ (small nuclear ribonucleoprotein polypeptides B and B1). It is located on human chromosome 20p13. SmB′/B are upregulated in all tissues excluding postnatal brain.

Immunogén

SmB recombinant protein.

Alkalmazás

• Monoclonal Anti-SmB antibody produced in mouse has been used in:
• western blotting
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation
• immunocytochemistry

Biokémiai/fiziológiai hatások

SNRPB (small nuclear ribonucleoprotein-associated protein B) mutations results in cerebro-costo-mandibular syndrome. Downregulation of SNRPB affects the upregulation and processing of RNA splicing machinery components. It also plays a major role in RNA stability.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)