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Sigma-Aldrich

Thrombin CleanCleave™ Kit

Szinonimák:

Thrombin Cleavage Kit

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About This Item

UNSPSC kód:
12352200
NACRES:
NA.56

form

suspension

Minőségi szint

200

molekulatömeg

37 kDa

technika/technikák

protein purification: suitable

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Általános leírás

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Alkalmazás

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Tulajdonságok és előnyök

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Mennyiség

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Jogi információk

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Kit Components Only

Product No.
Leírás
  • Thrombin Cleavage Buffer, 10× 10 mL

kapcsolódó termék

Product No.
Leírás
Árazás

08168

Timestrip Plus™ 8 °C

Tárolási osztály kódja

10 - Combustible liquids

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

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Journal of enzyme inhibition and medicinal chemistry, 35(1), 1834-1839 (2020-09-26)
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