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Merck

MAK329

Sigma-Aldrich

L-Lactate Assay Kit

sufficient for 100 colorimetric tests

Szinonimák:

L-Lactic Acid Assay Kit, Lactate Quantification Kit

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12161503
NACRES:
NA.84

használat

sufficient for 100 colorimetric tests

bemenet

cell
plasma
serum
solution(s) (collagen)

észlelési módszer

colorimetric

vonatkozó betegség(ek)

endocrinological disorders, diabetes; cancer

tárolási hőmérséklet

−20°C

Általános leírás

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable.

Alkalmazás

The L-Lactate Assay Kit may be used for:
  • Cancer Research
  • Diabetes Research
  • Endocrinological Disease Research

Tulajdonságok és előnyök

Sensitive Detection Range: Detect L-Lactate levels accurately across a wide range, from 0.05 mM to 2 mM, using only 20 μL of sample in a 96-well plate setup, ensuring highly sensitive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 20 minute room temperature reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Alkalmasság

Suitable for lactate determination in serum, plasma, and cell media samples.

Elv

The Lactate Assay Kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.

Egyéb megjegyzések

For additional information on our range of Biochemicals, please complete this form.

Tárolási osztály kódja

10 - Combustible liquids


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

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Bacteria equipped with genetically encoded lactate biosensors are promising tools for biopharmaceutical production, diagnostics, and cellular therapies. However, many applications involve glucose-rich and anoxic environments, in which current whole-cell lactate biosensors show low performance. Here we engineer an optimized, synthetic
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