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L3892

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

peroxidase conjugate, lyophilized powder

Szinonimák:

WGA, Wheat germ agglutinin

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About This Item

UNSPSC kód:
12352202
NACRES:
NA.32

biológiai forrás

Triticum vulgaris

Minőségi szint

200

konjugátum

peroxidase conjugate

Teszt

≥85% protein basis (Warburg-Christian)

form

lyophilized powder

hatékonyság

<40 μg/mL agglutination activity

peroxidase activity

50-200 units/mg protein

összetétel

Protein, ~90% modified Warburg-Christian

tárolási hőmérséklet

−20°C

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Alkalmazás

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biokémiai/fiziológiai hatások

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Kiszerelés

Package size based on protein content.

Egység definíció

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

Fizikai forma

Contains citrate buffer

Elkészítési megjegyzés

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Xinyu Qi et al.
Journal of bone and joint infection, 6(7), 241-253 (2021-07-16)
The high antibiotic tolerance of Staphylococcus aureus biofilms is associated with challenges for treating periprosthetic joint infection. The toxin-antitoxin system, YefM-YoeB, is thought to be a regulator for antibiotic tolerance, but its physiological role is unknown. The objective of this
Hugo Aguilar-Díaz et al.
PLoS neglected tropical diseases, 4(2), e607-e607 (2010-02-20)
Inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of Entamoeba histolytica under natural conditions. Unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available.
Qian Guo et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(10), 2065-2080 (2021-06-23)
ATP-citrate lyase (ACLY), generating most of the nucleocytosolic acetyl coenzyme A (acetyl-CoA) for histone acetylation, links cell metabolism to epigenetic regulation. Recent investigations demonstrated that ACLY activated by metabolic reprogramming played an essential role in both M1 and M2 macrophage
Qian Guo et al.
The Journal of biological chemistry, 101775-101775 (2022-03-09)
It's widely accepted that increasing mitochondrial respiration plays a pivotal role during osteoclastogenesis. Mitochondrial pyruvate carrier (MPC) is the key transporter that links glycolysis to mitochondrial respiration but little is known about its role during osteoclastogenesis. Our goal was to
Junlan Liu et al.
Communications biology, 4(1), 904-904 (2021-07-24)
Though a definitive link between small colony variants (SCVs) and implant-related staphylococcal infections has been well-established, the specific underlying mechanism remains an ill-explored field. The present study analyzes the role SCVs play in catheter infection by performing genomic and metabolic
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