Összes fotó(1)
Fontos dokumentumok
F2928
Monoclonal Anti-Fas Ligand antibody produced in rat
clone 101624, purified immunoglobulin, lyophilized powder
Szinonimák:
Anti-CD95L, Anti-FasL
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
konjugátum:
unconjugated
application:
Neutral
WB
WB
klón:
101624, monoclonal
faj reaktivitás:
mouse
citations:
6
technika/technikák:
neutralization: suitable
western blot: 1-2 μg/mL
western blot: 1-2 μg/mL
Javasolt termékek
biológiai forrás
rat
Minőségi szint
konjugátum
unconjugated
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
101624, monoclonal
Forma
lyophilized powder
faj reaktivitás
mouse
technika/technikák
neutralization: suitable
western blot: 1-2 μg/mL
izotípus
IgG1
tárolási hőmérséklet
−20°C
célzott transzláció utáni módosítás
unmodified
Géninformáció
mouse ... Fasl(14103)
Általános leírás
Fas Ligand is a 40kDa type II membrane protein, which also known as FasL and CD95L, belongs to the tumor necrosis factor (TNF) family. In the new TNF superfamily nomenclature, FasL is referred as TNFSF6. It consists of TNFα, α and β chains of lymphotoxin (LT), CD40 ligand, and CD30 ligand. It also has four potential N-glycosylation sites which appear to be variably used.
Immunogén
purified recombinant mouse Fas Ligand, amino acids 132-279, expressed in NSO cells.
Alkalmazás
The monoclonal Anti-Fas Ligand antibody is suitable for western blot analysis of rat thymus homogenates to detect the recombinant rFasL molecule. The antibody is also suitable for immunoblotting at a concentration of 1-2μg/ml and ELISA at a concentration of 0.5-1.0μg/ml.
Biokémiai/fiziológiai hatások
FasL is predominantly expressed on activated T cells and NK cells, whereas Fas is expressed on various cell types. The gene expression is induced by the activation of mature T cells with phorbol myristic acetate (PMA) and ionomycin, concanavalin A (Con A) or anti-CD3. After cleaving of FasL, it can generate the soluble Fas ligand, a non-covalently linked homotrimer. Membrane-bound FasL and TNFα are primary activators of their receptors. Soluble FasL may inhibit the killing effect of membrane FasL. It plays an important role in modulating immune response by inducing cell apoptosis to maintain homeostasis, self-tolerance of lymphocytes, and immune privilege. It has chemoattractant characteristics for neutrophils which indicate proinflammatory function.
Fizikai forma
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
13 - Non Combustible Solids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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S Sieg et al.
Journal of virology, 70(12), 8747-8751 (1996-12-01)
Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) are common human pathogens. In this report we demonstrate the capacity of HSV-2, but not HSV-1, to inhibit the activity and cell surface expression of Fas ligand, an important molecule
M G Cifone et al.
The Journal of experimental medicine, 180(4), 1547-1552 (1994-10-01)
Intracellular pathways leading from membrane receptor engagement to apoptotic cell death are still poorly characterized. We investigated the intracellular signaling generated after cross-linking of CD95 (Fas/Apo-1 antigen), a broadly expressed cell surface receptor whose engagement results in triggering of cellular
Gabriella Brunlid et al.
Stem cells (Dayton, Ohio), 25(10), 2551-2558 (2007-07-07)
The potential of pluripotent embryonic stem (ES) cells to develop into functional cells or tissue provides an opportunity in the development of new therapies for many diseases including neurodegenerative disorders. The survival of implanted cells usually requires systemic immunosuppression, however
T Suda et al.
Journal of immunology (Baltimore, Md. : 1950), 154(8), 3806-3813 (1995-04-15)
Fas ligand (FasL) is a membrane-type cytokine belonging to the TNF family, and induces apoptosis through its cell-surface receptor, Fas. To determine the cell types that express FasL, various mouse tissues and cell lines were examined by Northern hybridization using
A Oehm et al.
The Journal of biological chemistry, 267(15), 10709-10715 (1992-05-25)
The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 was previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. Cross-linking of the
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