LB58

98062325, human blood, Lymphoblastoid

• UNSPSC kód: 41106514
• biológiai forrás: human blood
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Lymphoblastoid
• termékek: Not specified
• receptorok: Not specified

Tulajdonságok

• Terméknév: LB58, 98062325
• biológiai forrás: human blood
• növekedési mód: Suspension
• kariotípus: Not specified
• morfológia: Lymphoblastoid
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human lymphoblastoid, Ataxia telangiectasia, EBV-transformed

Sejtvonal leírása

Established by EBV transformation of mononuclear cells from the peripheral blood of a 13 year old female with Ataxia telangiectasia (AT) complementation group C. AT is a rare autosomal recessive disorder. Lymphocytes have been shown to be hypersensitive to cytotoxic and clastogenic effects of gamma and X rays. T cells from the same original sample have been used for studies of mutant frequency at the hprt locus, with cells being described as Donor 58 or Patient 2 (see Lancet 1985). Fibroblasts derived from the same individual were reported to have RNA and genomic DNA sequence changes (del, 9nt).

Alkalmazás

hprt mutation studies, toxicity assays

Táptalaj

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Szubkultúra rutin

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Egyéb megjegyzések

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