6-23 (clone 6)

87042206, rat thyroid, Epithelial

• UNSPSC kód: 41106514
• biológiai forrás: rat thyroid
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Epithelial
• termékek: Neurotensin and calcitonin
• receptorok: Not specified
• vonatkozó betegség(ek): cancer

Tulajdonságok

• Terméknév: 6-23 (clone 6), 87042206
• biológiai forrás: rat thyroid
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Epithelial
• termékek: Neurotensin and calcitonin
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Rat medullary thyroid carcinoma

Sejtvonal leírása

Derived from the non-clonal line rMTC 6-23 originally established from a transplantable WAG/RIJ rat medullary thyroid carcinoma. The cell line synthesises and secretes both neurotensin and calcitonin. Its clonal derivation demonstrates that a population derived from a single type of C-cell has the capability of producing both peptides. Secretion of these peptides has been shown to be stimulated by calcium, KC1, glucagon and catecholamines.

Alkalmazás

Hormone studies

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Cells need to be grown on flasks coated with matrigel or any other attachment factor

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. For cell attachment culture vessels need to be coated with collagen type IV e.g. Sigma product C-5533. Soak in a sol

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