3T3 Swiss Albino

85022108, mouse embryo, Fibroblast

• UNSPSC kód: 41106514
• biológiai forrás: mouse embryo
• növekedési mód: Adherent
• kariotípus: 2n = 40; hypertriploid
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified

Tulajdonságok

• Terméknév: 3T3 Swiss Albino, 85022108
• biológiai forrás: mouse embryo
• növekedési mód: Adherent
• kariotípus: 2n = 40; hypertriploid
• morfológia: Fibroblast
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Általános leírás

Product has lost contact inhibition

Sejtvonal eredete

Mouse Swiss Albino embryo fibroblast

Sejtvonal leírása

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Alkalmazás

Contact inhibition and virus studies: polyoma and SV40 viruses

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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