C-39365

Skeletal Muscle Cell Growth Medium

SupplementMix containing all media supplements pre-mixed in one vial, 1 Mix for 500 ml

• Szinonimák: Recommended with C-23260
• UNSPSC kód: 12352207
• NACRES: NA.71

Tulajdonságok

• form: aqueous solution
• kiszerelés: pkg of 1 vial
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Skeletal Muscle Cell Growth Medium SupplementMix (basal medium sold separately)
To form a complete medium, it should be combined with the basal medium: C-23260

Alkalmazás

Skeletal Muscle Cell Growth Medium is a low-serum medium (5% V/V) developed for the expansion of human skeletal muscle cell cultures. The media are optimized for primary human cells, but can also be used for murine, rat, canine, and rabbit skeletal muscle cells.Recommended for:Human Skeletal Muscle Cells (SkMC)

Komponensek

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Minőség

All lots of PromoCell Skeletal Muscle Cell Growth Medium are subjected to comprehensive quality control tests using human primary SkMC. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested SkMC. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Elkészítési megjegyzés

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / mlFetuin (bovine): 50 µg / mlEpidermal Growth Factor (recombinant human): 10 ng / mlBasic Fibroblast Growth Factor (recombinant human): 1 ng / mlInsulin (recombinant human): 10 µg / mlDexamethasone: 0.4 µg / ml

Tárolás és stabilitás

Store the SupplementMix at -20°C in the dark immediately after arrival. If stored properly, it is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Szubkultúra rutin

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Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable