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Merck
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C-12907

Sigma-Aldrich

Human Mononuclear Cells (hMNC)

Isolated from peripheral blood, single donor, ultra-pure, 25 million cryopreserved cells

Szinonimák:

hMNC cells

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106514
NACRES:
NA.81

biológiai forrás

human peripheral blood

kiszerelés

pkg of 25,000,000 cells

morfológia

(mononuclear)

technika/technikák

cell culture | mammalian: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−196°C

Általános leírás

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Sejtvonal eredete

Blood

Alkalmazás

Mononuclear Cells (MNC) represent the lymphocyte and monocyte fraction isolated from peripheral blood. They are prepared by optimized low-density gradient centrifugation, which effectively reduces the granulocyte content. Red blood cells are gently but thoroughly depleted by proprietary techniques instead of osmotic lysis. This results in ultra-pure mononuclear cells with superior viability and unchanged biological function. In addition, the cells do not clump after thawing, allowing for easy handling and reproducible results.The MNC can be used directly for experiments or as a starting material for the isolation of several cell types, e.g., hematopoietic and endothelial progenitors, monocytes, and lymphocyte subpopulations.

Minőség

Rigid quality control tests are performed for each lot of Mononuclear Cells. They are routinely characterized by flow cytometry analyzing a series of cellular parameters, e.g. viability, cell size, granularity. provides detailed information on the percentages of the major cellular sub-populations, i.e., the lymphocytes, the monocytes, and granulocytes, for each lot of Mononuclear Cells. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Figyelmeztetés

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Szubkultúra rutin

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Egyéb megjegyzések

Recommended Plating Density: 1 x 106 cells per mlTested Markers: CD31 negative, CD34 negative, CD105 positive, CD146 positive

Jogi nyilatkozat

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


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Cikkek

Umbilical cord blood and adult peripheral blood mononuclear cells with optimized maintenance medium for short-term cultivation.

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