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Merck
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C-12640

Sigma-Aldrich

Human Bronchial Epithelial Cells (HBEpC)

500,000 cryopreserved cells

Szinonimák:

HBEpC cells

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106514
NACRES:
NA.81

biológiai forrás

human bronchi

kiszerelés

pkg of 500,000 cells

morfológia

(epithelial)

technika/technikák

cell culture | mammalian: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−196°C

Általános leírás

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Sejtvonal eredete

Bronchi

Alkalmazás

Primary Human Bronchial Epithelial Cells (HBEpC) are isolated from the surface epithelium of human bronchi and are provided in a cryopreserved format. HBEpC stain positive for cytokeratin. The respiratory epithelia are responsible for the lubrication of the lungs, the maintenance of humidity, and the cleaning of the respiratory tract. They are an important target for drugs, toxins, and carcinogens. In addition, they are involved in many diseases such as cystic fibrosis. Consequently, HBEpC are useful for investigating the function and pathology of the respiratory system.

Minőség

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Figyelmeztetés

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Szubkultúra rutin

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Egyéb megjegyzések

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Javasolt termékek

Recommended Primary Cell Culture Media:Link

Jogi nyilatkozat

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Annika Hunziker et al.
Cell reports, 38(4), 110306-110306 (2022-01-27)
Binding of influenza virus to its receptor triggers signaling cascades that reprogram the cell for infection. To elucidate global virus-induced changes to the cellular signaling landscape, we conducted a quantitative phosphoproteomic screen with human and avian influenza viruses. Proteins with

Cikkek

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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