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Merck
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Fontos dokumentumok

C-12620

Sigma-Aldrich

Human Nasal Epithelial Cells (HNEpC)

500,000 cryopreserved cells

Szinonimák:

HNEpC cells

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106514
NACRES:
NA.81

biológiai forrás

human nose (nasal mucosa)

kiszerelés

pkg of 500,000 cells

morfológia

(epithelial)

technika/technikák

cell culture | mammalian: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−196°C

Általános leírás

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Sejtvonal eredete

Nose

Alkalmazás

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and are provided in a cryopreserved format. HNEpC stain positive for cytokeratin. The epithelium in the nasal cavity cleans, humidifies, and warms inhaled air. In addition, it produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. HNEpC are useful for in vitro studies of these processes.

Minőség

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Figyelmeztetés

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Szubkultúra rutin

Click here for more information.

Egyéb megjegyzések

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Javasolt termékek

Recommended Primary Cell Culture Media:Link

Jogi nyilatkozat

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Govind Gupta et al.
Frontiers in bioengineering and biotechnology, 10, 1083232-1083232 (2022-12-30)
Silver (Ag) is known to possess antimicrobial properties which is commonly attributed to soluble Ag ions. Here, we showed that Ag nanoparticles (NPs) potently inhibited SARS-CoV-2 infection using two different pseudovirus neutralization assays. We also evaluated a set of Ag

Cikkek

Optimize primary human airway epithelial cell culture with serum-free media and handling protocols for large air passage cells.

Yeast plasmid selection: Utilizes auxotrophic yeast strains for plasmid selection, enabling growth on synthetic media for better yield.

Overview of primary cells and media usage in biomedical culture applications highlights differences from cell lines.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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